||Recombinant humanMAGEA6 protein, fused to His-tag at C-terminus, was expressed in E.coli andpurified by using conventional chromatography techniques.
||MAGEA6 is a memberof the MAGEA family, which directs the expression of tumor antigens that arerecognized on a human melanoma by autologous cytolytic T lymphocytes (CTL).The MAGE genes were initially isolated from different kinds of tumors, andbased on their virtually exclusive tumor-specific expression in adulttissues, they have been used as targets for cancer immunotherapy
||Liquid. In 20mMTris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol, 1mM DTT
||35.9 kDa (322aa),confirmed by MALDI-TOF
||> 90% by SDS -PAGE
||1 mg/ml (determinedby Bradford assay)
|Sequences of aminoacids:
||MPLEQRSQHCKPEEGLEARG EALGLVGAQA PATEEQEAAS SSSTLVEVTL GEVPAAESPD PPQSPQGASS LPTTMNYPLWSQSYEDSSNQ EEEGPSTFPD LESEFQAALS RKVAKLVHFL LLKYRAREPV TKAEMLGSVV GNWQYFFPVIFSKASDSLQL VFGIELMEVD PIGHVYIFAT CLGLSYDGLL GDNQIMPKTG FLIIILAIIA KEGDCAPEEKIWEELSVLEV FEGREDSIFG DPKKLLTQYF VQENYLEYRQ VPGSDPACYE FLWGPRALIE TSYVKVLHHMVKISGGPRIS YPLLHEWALR EGEELEHHHH HH
||Can be stored at+4°C short term (1-2 weeks). For long term storage, aliquot and store at-20°C or -70°C. Avoid repeated freezing and thawing cycles.