||Recombinant humanIFNG protein,fused to His-tag at N-terminus, was expressed in E. coli andpurified by using conventional chromatography.
||IFNG, also known as interferon gamma, is secreted by lymphocytes stimulatedby mitogen and is involved in the differentiation, maturation, andproliferation of hematopoietic cells. Nature human IFNG is composed of 143amino acid residues without cysteine residues and is glycosylated.Proteolytic processing produces C-terminal heterogeneity, with proteinsending alternatively at Gly-150, Met-157 or Gly-161.
||Liquid.Phosphate buffered saline (pH 7.4).
||18.5 kDa (159aa)confirmed by MALDI-TOF
||> 90% by SDS-PAGE
||1 mg/ml(determined by Bradford assay)
|Sequences of aminoacids:
||MGSSHHHHHH SSGLVPRGSH MQDPYVKEAE NLKKYFNAGHSDVADNGTLF LGILKNWKEE SDRKIMQSQI VSFYFKLFKN FKDDQSIQKS VETIKEDMNV KFFNSNKKKRDDFEKLTNYS VTDLNVQRKA IHELIQVMAE LSPAAKTGKR KRSQMLFRG
||Can bestored at +4°C short term (1-2 weeks). For long term storage, aliquot andstore at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
|Pathway||Allograft rejection; Cytokine-cytokine receptor interaction;Graft-versus-host disease; Jak-STAT signaling pathway; Natural killer cellmediated cytotoxicity; Proteasome; Regulation of autophagy; Systemic lupuserythematosus; T cell receptor signaling pathway; TGF-beta signaling pathway;Type I diabetes mellitus|