||Recombinant mouse Il1rnprotein, fused to His-tag at N-terminus was expressed in E. coli and purifiedby using conventional chromatography techniques.
||Interleukin1 receptor antagonist (Il1rn), alsoknown as IL1-RA, is a member of the interleukin 1 cytokine family. IL1RN mayrepresent a reservoir of IL-1 inhibitors, released upon cell death, whosefunction is no limit the pro-inflammatory signals but most prominently bymononuclear phagocytes and keratinocytes. IL1RN is reported to be associatedwith increased risk of osteoporotic fractures and gastric cancer.
||Liquid. In 20mMTris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol.
||20.0 kDa(177aa), confirmed by MALDI-TOF.
||> 95%by SDS-PAGE
||1 mg/ml(determined by Bradford assay)
|Sequences of aminoacids:
||MGSSHHHHHHSSGLVPRGSH MGSHMRPSGK RPCKMQAFRI WDTNQKTFYL RNNQLIAGYL QGPNIKLEEKIDMVPIDLHS VFLGIHGGKL CLSCAKSGDD IKLQLEEVNI TDLSKNKEEDKRFTFIRSEK GPTTSFESAA CPGWFLCTTL EADRPVSLTN TPEEPLIVTK FYFQEDQ
||Can bestored at +4°C short term (1-2 weeks). For long term storage, aliquot andstore at -20°C or -70°C. Avoid repeated freezing and thawing cycles.