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Rabbit Anti-Human Epidermal Growth Factor Monoclonal Antibody

Cat.No. : CAB11526RH
Product Overview : Rabbit Monoclonal Antibody to Human Epidermal Growth Factor
  • Specification
  • Gene Information
  • Related Products
Antigen Description : Human epidermal growth factor ( EGF ) exerts actions on a wide variety of biological processes, and plays an important role in the regulation of cell growth, proliferation, and differentiation mediated by its high-affinity receptor EGFR on the cell surface. It induces differentiation of specific cells, promotes angiogenesis, and inhibits gastric acid secretion in vivo, while serves as a potent mitogenic actor for a variety of cultured cells of both ectodermal and mesodermal origin. Human EGF was originally isolated from urine based on its inhibitory effect on gastric secretion and named urogastrone accordingly. EGF is the founding member of the EGF-family of proteins which are characterized by the presence of at least one EGF structural unit (a conserved 6 cysteine motif that forms three disulfide bonds). The EGF precursor is believed to exist as a membrane-bound molecule containing 9 EGF units, and is proteolytically cleaved to generate the 53-amino acid peptide hormone.
Specificity : Human Epidermal Growth Factor. No cross-reactivity with human cell lysate ( 293 cell line ) in WB and ELISA.
Immunogen : Recombinant human EGF protein
Host : Rabbit
Isotype : Rabbit IgG
Species : Human
Clone : A009
Applications : WB; ELISA
Dilution : Western blot: This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect human human EGF in WB. Using a DAB detection system, the detection limit for human EGF is approximately 0.5 ng/lane under non-reducing conditions and 20 ng/lane under reducing conditionsELISA: This antibody can be used at 0.1 - 0.2 μg/mL with the appropriate secondary reagents to detect human EGF. The detection limit for human EGF is approximately 0.0049 ng/well
Preparation : This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant human EGF
Format : 0.2 μm filtered solution in PBS with 5% trehalose
Storage : This antibody can be stored at 2-8 °C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 to -70°C. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Gene Name : EGF epidermal growth factor [ Homo sapiens ]
Official Symbol : EGF
Synonyms : EGF; epidermal growth factor; epidermal growth factor (beta urogastrone); pro-epidermal growth factor; beta-urogastrone; URG; HOMG4;
Gene ID : 1950
mRNA Refseq : NM_001178130
Protein Refseq : NP_001171601
MIM : 131530
UniProt ID : P01133
Chromosome Location : 4q25
Pathway : Arf6 signaling events, organism-specific biosystem; Bladder cancer, organism-specific biosystem; Bladder cancer, conserved biosystem; Ceramide signaling pathway, organism-specific biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Delta-Notch Signaling Pathway, organism-specific biosystem;
Function : calcium ion binding; epidermal growth factor receptor binding; growth factor activity; protein binding; transmembrane receptor protein tyrosine kinase activator activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (5)

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How is EGF protein produced in the body? 02/07/2022

The body naturally produces EGF protein, and it can also be synthesized for clinical applications using biotechnological methods.

Are there any side effects associated with the clinical use of EGF protein? 12/06/2021

Generally, EGF is well-tolerated, but localized irritation or redness at the application site can occur in some cases.

Can EGF be used in the treatment of burns? 11/03/2021

Yes, EGF has been explored for its potential in treating burns by promoting the regeneration of damaged skin tissue.

What are the primary clinical applications of EGF protein? 10/19/2021

EGF protein is widely used in clinical settings for wound healing, tissue regeneration, and certain dermatological conditions.

Is EGF used in cosmetic products, and if so, for what purpose? 09/28/2019

Yes, EGF is a common ingredient in some cosmetic products due to its ability to promote skin rejuvenation and reduce the appearance of fine lines and wrinkles.

Customer Reviews (3)

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Reviews
01/27/2020

    This can be advantageous in understanding disease mechanisms and developing novel therapeutics.

    04/12/2018

      The manufacturer can provide detailed documentation on the quality control measures undertaken during protein production and purification.

      05/09/2016

        The manufacturer can offer technical expertise and guidance to researchers regarding the proper use, handling, and storage of EGF protein.

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