Recombinant Mouse Interleukin 18, His-tagged
Cat.No. : | Il18-740M |
Product Overview : | Recombinant Mouse Il18 was expressed as His-tagged fusion protein in E. coli and purifed by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer. MW: 20.4 kDa. |
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Description : | Il18, previously known as interferon-gamma (IFN-gamma)-inducing factor, is an activator of NK cells and a co-inducer of Th1 cytokines. IL-18 is produced from Kupffer cells, activated macrophages, keratinocytes, intestinal epithelial cells, osteoblasts, adrenal cortex cells and murine diencephalon. IL-18 was expressed in E. coli and purifed by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer. |
Source : | E. coli |
Species : | Mouse |
Tag : | His |
Form : | In 20mM Tris-HCl pH 8.0 containing 10% glycerol |
Molecular Mass : | 20.4 kDa (178 aa), confirmed by MALDI-TOF. |
Protein length : | 36-192aa |
AA Sequence : | MGSSHHHHHH SSGLVPRGSH MNFGRLHCTT AVIRNINDQV LFVDKRQPVF EDMTDIDQSA SEPQTRLIIY MYKDSEVRGL AVTLSVKDSK MSTLSCKNKI ISFEEMDPPE NIDDIQSDLI FFQKRVPGHN KMEFESSLYE GHFLACQKED DAFKLILKKK DENGDKSVMF TLTNLHQS |
Endotoxin : | < 1.0 eu per 1 μg of protein (determined by lal |
Purity : | >90% by SDS - PAGE |
Applications : | SDS-PAGE |
Storage : | Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles. |
Concentration : | 1 mg/ml (determined by Bradford assay) |
Gene Name : | Il18 interleukin 18 [ Mus musculus ] |
Official Symbol : | Il18 |
Synonyms : | IL18; interleukin 18; interleukin-18; IL-1 gamma; interleukin-1 gamma; IFN-gamma-inducing factor; interferon gamma-inducing factor; interferon-gamma-inducing factor; Igif; Il-18; |
Gene ID : | 16173 |
mRNA Refseq : | NM_008360 |
Protein Refseq : | NP_032386 |
Pathway : | African trypanosomiasis, organism-specific biosystem; African trypanosomiasis, conserved biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Cytosolic DNA-sensing pathway, organism-specific biosystem; Cytosolic DNA-sensing pathway, conserved biosystem; Hypertrophy Model, organism-specific biosystem; |
Function : | cytokine activity; |
Products Types
◆ Recombinant Protein | ||
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IL18-1012H | Recombinant Human IL18 Protein, His-tagged | +Inquiry |
IL18-2057R | Recombinant Rhesus Macaque IL18 Protein, His (Fc)-Avi-tagged | +Inquiry |
Il18-155M | Recombinant Active Mouse IL18 Protein, His-tagged(C-ter) | +Inquiry |
◆ Lysates | ||
IL18-344HCL | Recombinant Human IL18 lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (10)
Ask a questionCo-immunoprecipitation, co-localization studies, and pathway inhibition assays decipher IL18's interactions with signaling molecules and pathways.
Therapeutic antibody trials, in vitro drug screens, and disease model assessments assess IL18-targeted interventions in autoimmune diseases.
Xenograft models, intravital imaging, and tissue-specific knockout approaches unravel IL18's kinetics and effects within complex in vivo environments.
Flow cytometry, chemotaxis assays, and functional cell assays examine IL18's impact on immune cell activation, migration, and effector functions.
Surface plasmon resonance, mutational studies, and phosphoproteomics illuminate IL18's receptor interactions and downstream signal transduction pathways.
Immunophenotyping, cytokine profiling, and gene expression analyses elucidate IL18's influence on Th cell polarization and immune balance.
ChIP assays, promoter analyses, and epigenetic profiling uncover the regulatory mechanisms underlying IL18 gene expression dynamics.
Disease models, knockout mice, and co-stimulation studies explore IL18's involvement in inflammatory conditions and its interplay with other proinflammatory mediators.
Tumor models, immune profiling, and immunotherapeutic studies investigate IL18's modulation of the tumor microenvironment and its implications for cancer therapy.
Caspase assays, cytokine ELISAs, and microscopy techniques unveil the activation of inflammasomes and IL18's contribution to pyroptosis and cytokine storms.
Customer Reviews (3)
Write a reviewEffective in inflammation models.
High stability in serum.
Suitable for ELISA.
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