Recombinant Mouse Interleukin 18, His-tagged Il18-740M

Recombinant Mouse Interleukin 18, His-tagged

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Recombinant Mouse Interleukin 18, His-tagged

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Cat.No. : Il18-740M
Product Overview : Recombinant Mouse Il18 was expressed as His-tagged fusion protein in E. coli and purifed by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer. MW: 20.4 kDa.
Description : Il18, previously known as interferon-gamma (IFN-gamma)-inducing factor, is an activator of NK cells and a co-inducer of Th1 cytokines. IL-18 is produced from Kupffer cells, activated macrophages, keratinocytes, intestinal epithelial cells, osteoblasts, adrenal cortex cells and murine diencephalon. IL-18 was expressed in E. coli and purifed by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
Source : E. coli
Species : Mouse
Tag : His
Form : In 20mM Tris-HCl pH 8.0 containing 10% glycerol
Molecular Mass : 20.4 kDa (178 aa), confirmed by MALDI-TOF.
Protein length : 36-192aa
AA Sequence : MGSSHHHHHH SSGLVPRGSH MNFGRLHCTT AVIRNINDQV LFVDKRQPVF EDMTDIDQSA SEPQTRLIIY MYKDSEVRGL AVTLSVKDSK MSTLSCKNKI ISFEEMDPPE NIDDIQSDLI FFQKRVPGHN KMEFESSLYE GHFLACQKED DAFKLILKKK DENGDKSVMF TLTNLHQS
Endotoxin : < 1.0 eu per 1 μg of protein (determined by lal
Purity : >90% by SDS - PAGE
Applications : SDS-PAGE
Storage : Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Concentration : 1 mg/ml (determined by Bradford assay)
Gene Name : Il18 interleukin 18 [ Mus musculus ]
Official Symbol : Il18
Synonyms : IL18; interleukin 18; interleukin-18; IL-1 gamma; interleukin-1 gamma; IFN-gamma-inducing factor; interferon gamma-inducing factor; interferon-gamma-inducing factor; Igif; Il-18;
Gene ID : 16173
mRNA Refseq : NM_008360
Protein Refseq : NP_032386
Pathway : African trypanosomiasis, organism-specific biosystem; African trypanosomiasis, conserved biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Cytosolic DNA-sensing pathway, organism-specific biosystem; Cytosolic DNA-sensing pathway, conserved biosystem; Hypertrophy Model, organism-specific biosystem;
Function : cytokine activity;

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