Recombinant Human POFUT1, His-tagged

• Cat.No.: POFUT1-7213H

Specification

• product overview: Recombinant human POFUT1 protein, fused to His-tag at N-terminus, was expressed in E.coli.
• description: GDP-fucose protein O-fucosyltransferase 1, also known as POUFUT1, is a member of the glycosyltransferase O-Fuc family. Highly expressed in pancreas, kidney, lung, heart, brain, liver, placenta and skeletal muscle, POFUT1 uses manganese to catalyze the attachment of fucose to a conserved serine or threonine residue on a protein accept. POFUT1 plays an important role in Notch signaling, as Notch ligands can serve as POFUT1 substrates.
• source: E. coli
• species: Human
• tag: His
• form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 2M Urea, 10% glycerol
• molecular mass: 43.7kDa (385aa)
• protein length: 27-388aa
• aa sequence: MGSSHHHHHH SSGLVPRGSH MGSGSWDPAG YLLYCPCMGR FGNQADHFLG SLAFAKLLNR TLAVPPWIEY QHHKPPFTNL HVSYQKYFKL EPLQAYHRVI SLEDFMEKLA PTHWPPEKRV AYCFEVAAQR SPDKKTCPMK EGNPFGPFWD QFHVSFNKSE LFTGISFSAS YREQWSQRFS PKEHPVLALP GAPAQFPVLE EHRPLQKYMV WSDEMVKTGE AQIHAHLVRP YVGIHLRIGS DWKNACAMLK DGTAGSHFMA SPQCVGYSRS TAAPLTMTMC LPDLKEIQRA VKLWVRSLDA QSVYVATDSE SYVPELQQLF KGKVKVVSLK PEVAQVDLYI LGQADHFIGN CVSSFTAFVK RERDLQGRPS SFFGMDRPPK LRDE
• purity: >85% by SDS - PAGE
• applications: SDS-PAGE
• storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• concentration: 1 mg/ml

Gene Information

• gene name: POFUT1 protein O-fucosyltransferase 1 [ Homo sapiens ]
• official symbol: POFUT1
• synonyms: POFUT1; protein O-fucosyltransferase 1; GDP-fucose protein O-fucosyltransferase 1; FUT12; KIAA0180; O Fuc T; O FUT; o-fucosyltransferase protein; peptide-O-fucosyltransferase 1; O-FUT; O-Fuc-T; O-FucT-1; MGC2482
• Gene ID: 23509
• mrna refseq: NM_015352
• protein refseq: NP_056167
• mim: 607491
• uniprot id: Q9H488
• chromosome location: 20q11
• pathway: Delta-Notch Signaling Pathway, organism-specific biosystem; Other types of O-glycan biosynthesis, organism-specific biosystem; Other types of O-glycan biosynthesis, conserved biosystem; Pre-NOTCH Expression and Processing, organism-specific biosystem; Pre-NOTCH Processing in the Endoplasmic Reticulum, organism-specific biosystem; Signal Transduction, organism-specific biosystem; Signaling by NOTCH, organism-specific biosystem
• function: fucosyltransferase activity; peptide-O-fucosyltransferase activity; transferase activity, transferring glycosyl groups