| Background : |
CD4 (cluster of differentiation 4) is a glycoprotein expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells. It was discovered in the late 1970s and was originally known as leu-3 and T4 (after the OKT4 monoclonal antibody that reacted with it) before being named CD4 in 1984. In humans, the CD4 protein is encoded by the CD4 gene. |
| Cell Line Description : |
The antibody is directed against B°CD4 antigen, the bovine equivalent of human CD4 found on helper/inducer T cells. |
| Depositor : |
Dr J Naessens, ILRAD, Nairobi, Kenya |
| Immunogen : |
Bovine alloreactive lymphocytes |
| Immunological Donor : |
BALB/c mouse spleen |
| Fusion Species : |
Mouse X Mouse Hybridoma |
| Myeloma : |
P3x63Ag8 |
| Growth properties : |
Suspension |
| Propagation : |
Complete growth medium: RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS) Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0 °C |
| Subculturing : |
Maintain cultures between 3-9x10^5 cells/ml; 5% CO2; 37 °C. |
| Mycoplasma : |
Mycoplasma Status: Negative (MycoAlert Kit) |
| Reactivity : |
Anti-CD4, bovine |
| Isotype : |
IgG2a |
| Target : |
CD4 |
| Application : |
Enumeration of bovine lymphocyte populations |
| Storage : |
Liquid nitrogen |
| Safety Considerations : |
The following safety precautions should be observed.1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.2. No eating, drinking or smoking while handling the hybridoma.3. Wash hands after handling the stable line and before leaving the lab.4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with hybridoma.5. All waste should be considered hazardous.6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship : |
Dry ice |
