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Recombinant Human IL1F5

Cat. No.: IL1F5-316H
Product Overview: Recombinant Human IL-1F5 produced in E. coli is a single, non-glycosylated, polypeptide chain containing 155 amino acids and having a molecular mass of 17 kDa.
Description: Human interleukin family 1, member 5 (IL-1F5 / FIL1-delta) is a member of the interleukin 1 cytokine family. IL-1F5 has been reported to be expressed by various cells including monocytes, Bcells, dendritic cells/Langerhans cells, keratinocytes,and gastric fundus Parietal and Chief cells. IL-1F5 was reported to be an antagonist of IL-1F9, however IL-1F5 activity associated with receptor binding remains unclear. Human and mouse IL-1F5 share 90% a.a. sequence identity.
Biological Activity: As measured by its binding ability in a functional ELISA, immobilized rHuIL-1F5 at 1 μg/ml (100 μl/well) can bind rHuIL-1 Rrp2/Fc Chimera with a linear range of 0.15~5 μg/ml.
Purity: Greater than 95% as determined by (a) Analysis by RP-HPLC; (b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
Amino-Acid Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Val-Leu-Ser-Gly.
Endotoxin: Less than 0.1ng/µg (1 IEU/µg) determined by LAL test.
Form: The protein was lyophilized after extensive dialysis against 20mM Phosphate buffer, pH7.4.
Reconstitution: It is recommended to quick spin followed by reconstitution of rHuIL-1F5 in PBS to a concentration no less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Storage: Lyophilized rHuIL-1F5 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution rHuIL-1F5 should be stored at 4°C between 2-7 days and for future use below -18°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.
Gene Name: IL1F5 interleukin 1 family, member 5 (delta) [ Homo sapiens ]
Official Symbol: IL1F5
Synonyms: IL1F5; interleukin 1 family, member 5 (delta); FIL1; FIL1D; IL1L1; IL1HY1; IL1RP3; MGC29840; FIL1(DELTA) ; interleukin-1 family member 5 ; IL-1L1 ; IL-1HY1 ; FIL1 delta ; IL-1 delta ; IL-1ra homolog 1 ; interleukin-1 HY1; OTTHUMP00000162015; OTTH- UMP00000203697; OTTHUMP00000203698; interleuk- in-1 delta; interleukin 1, delta ; IL-1 related protein 3 ; IL-1-related protein 3 ; interleukin-1-like protein 1 ; family of interleukin 1-delta ; IL1F5 (Canonical product IL-1F5a) ; interleukin-1 receptor antagonist homolog 1; IL-1F5 (IL-1HY1, FIL1-delta, IL-1RP3, IL-1L1, IL-1-delta) ; Interleukin-1 HY1 ; Interleukin-1 receptor antagonist homolog 1 ; Interleukin-1-like protein 1 ; Interleukin-1 delta
Gene ID: 26525
mRNA Refseq: NM_012275
Protein Refseq: NP_036407
MIM: 605507
UniProt ID: Q9UBH0
Chromosome Location: 2q14
Function: cytokine activity; interleukin-1 receptor antagonist activity

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