| Cat. No.: | IL1F5-316H |
| Product Overview: | Recombinant Human IL-1F5 produced in E. coli is a single, non-glycosylated, polypeptide chain containing 155 amino acids and having a molecular mass of 17 kDa. |
| Description: | Human interleukin family 1, member 5 (IL-1F5 / FIL1-delta) is a member of the interleukin 1 cytokine family. IL-1F5 has been reported to be expressed by various cells including monocytes, Bcells, dendritic cells/Langerhans cells, keratinocytes,and gastric fundus Parietal and Chief cells. IL-1F5 was reported to be an antagonist of IL-1F9, however IL-1F5 activity associated with receptor binding remains unclear. Human and mouse IL-1F5 share 90% a.a. sequence identity. |
| Biological Activity: | As measured by its binding ability in a functional ELISA, immobilized rHuIL-1F5 at 1 μg/ml (100 μl/well) can bind rHuIL-1 Rrp2/Fc Chimera with a linear range of 0.15~5 μg/ml. |
| Purity: | Greater than 95% as determined by (a) Analysis by RP-HPLC; (b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. |
| Amino-Acid Sequence: | The sequence of the first five N-terminal amino acids was determined and was found to be Met-Val-Leu-Ser-Gly. |
| Endotoxin: | Less than 0.1ng/µg (1 IEU/µg) determined by LAL test. |
| Form: | The protein was lyophilized after extensive dialysis against 20mM Phosphate buffer, pH7.4. |
| Reconstitution: | It is recommended to quick spin followed by reconstitution of rHuIL-1F5 in PBS to a concentration no less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Storage: | Lyophilized rHuIL-1F5 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution rHuIL-1F5 should be stored at 4°C between 2-7 days and for future use below -18°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles. |
| Gene Name: | IL1F5 interleukin 1 family, member 5 (delta) [ Homo sapiens ] |
| Official Symbol: | IL1F5 |
| Synonyms: | IL1F5; interleukin 1 family, member 5 (delta); FIL1; FIL1D; IL1L1; IL1HY1; IL1RP3; MGC29840; FIL1(DELTA) ; interleukin-1 family member 5 ; IL-1L1 ; IL-1HY1 ; FIL1 delta ; IL-1 delta ; IL-1ra homolog 1 ; interleukin-1 HY1; OTTHUMP00000162015; OTTH- UMP00000203697; OTTHUMP00000203698; interleuk- in-1 delta; interleukin 1, delta ; IL-1 related protein 3 ; IL-1-related protein 3 ; interleukin-1-like protein 1 ; family of interleukin 1-delta ; IL1F5 (Canonical product IL-1F5a) ; interleukin-1 receptor antagonist homolog 1; IL-1F5 (IL-1HY1, FIL1-delta, IL-1RP3, IL-1L1, IL-1-delta) ; Interleukin-1 HY1 ; Interleukin-1 receptor antagonist homolog 1 ; Interleukin-1-like protein 1 ; Interleukin-1 delta |
| Gene ID: | 26525 |
| mRNA Refseq: | NM_012275 |
| Protein Refseq: | NP_036407 |
| MIM: | 605507 |
| UniProt ID: | Q9UBH0 |
| Chromosome Location: | 2q14 |
| Function: | cytokine activity; interleukin-1 receptor antagonist activity |
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