Active Recombinant Mouse Il36rn Protein, His-Tagged

• Cat.No.: Il36rn-01M
• Product Overview: Recombinant mouse Il36rn Protein, His-tagged was expressed in E. coli cell

Specification

• Description: As a result of the interaction of IL-36 ligands with the IL-1Rrp2 receptor. Various activities including dendritic cell maturation and activation are induced. IL-36RA can antagonize the NF-κB signaling through binding to the IL-1Rrp2 receptor by either IL-36α, β or γ in a way of preventing the initiation of functional signaling.
• Source: Escherichia coli
• Species: Mouse
• Tag: His
• Form: Lyophilized powder
• AA Sequence: MVLSGALCFRMKDSALKVLYLHNNQ LLAGGLHAEKVIKGEEISVVPNRAL DASLSPVILGVQGGSQCLSCGTEKG PILKLEPVNIMELYLGAKE
  SKSFTFYRRDMGLTSSFESAAYPGW FLCTSPEADQPVRLTQIPEDPAWDA PITDFYFQQCD with polyhistidine tag at the C-terminus
• Endotoxin: <0.1 EU per 1 μg of the protein by the LAL method.
• Bio-activity: Measure by its ability to inhibit IL-36 gamma-induced IL-6 secretion in 3T3 cells. The ED50 for this effect is <2 μg/mL.
• Purity: >98% as determined by SDS-PAGE. Ni-NTA chromatography
• Storage Buffer: The protein was lyophilized from a solution containing 1X PBS, pH 7.4.
• Reconstitution: It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 100 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
• Storage: Lyophilized protein should be stored at -20°C. Upon reconstitution, protein aliquots should be stored at -20°C or -80°C.
• Notes: Please use within one month after protein reconstitution.

Gene Information

• Gene Name: Il36rn interleukin 36 receptor antagonist [ Mus musculus (house mouse) ]
• Official Symbol: Il36rn
• Synonyms: Il1f5; If36rn; Il-1h3; Il1hy1; IL-36Ra; Fil1delta
• Gene ID: 54450
• mRNA Refseq: NM_001146087.1
• Protein Refseq: NP_001139559.1
• UniProt ID: Q9JIG2

Reviews

05/31/2021

When conducting experiments with IL36RN, clear and consistent results can be obtained, facilitating data analysis and interpretation.

01/29/2019

IL36RN has high specific activity and can exert strong biological effect at low concentration.

01/11/2019

The stability of the IL36RN product allows it to cope with different experimental conditions and modes of operation.

Q&As

What is the action mechanism of IL36RN?

The mechanism of action of IL36RN is to play an anti-inflammatory, anti-infection, anti-tumor and other biological mechanisms by acting on the signaling pathway of IL-36 receptor.

How to ensure the purity of IL36RN when preparing it?

The purity of IL36RN can be ensured by ion exchange chromatography, dialysis, gel filtration and other methods.

IL36RN production process is what?

Mass production of IL36RN can be carried out through large-scale fermentation, purification and other processes.

What is the difference between IL36RN protein and IL36α, β, γ?

This protein is an inhibitor with no biological activity of its own; Recombinant IL36α, β and γ are agonists with different biological effects.

Can recombinant IL36RN be used in clinical trials and therapeutics?

Can be used in clinical trials and therapy.

Does IL36RN have sustained and stable biological activity?

IL36RN has sustained and stable biological activity.