Recombinant Human NGF

PRODUCTS

Home / Products / Recombinant Proteins / Recombinant Human NGF

Recombinant Human NGF

NGF Related Products

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.

Cat. No. : NGF-495H
Product Overview : Recombinant Human Beta-NGF produced in CHO cells is a noncovalently disulfide-linked homodimer, of two 118 amino acids polypeptide monomers and having a molecular mass of 26.5 kDa.
Description : Beta-NGF is a neurotrophic factor structurally related to BDNF, NT-3 and NT-4. These proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures. Beta-NGF is a potent neurotrophic factor that signals through its receptor beta-NGFR, and plays a crucial role in the development and preservation of the sensory and sympathetic nervous systems. Beta-NGF also acts as a growth and differentiation factor for B lymphocytes and enhances B-cell survival. The functional form of human beta-NGF is a homodimer and the three disulfide bonds are required for biological activity.
Biological Activity : The ED50 as determined by its ability to stimulate chick E9 DRG neurite outgrowth was found to be < 1 ng/ml, corresponding to a Specific Activity of 1.0 x 10⁶ IU/mg.
Purity : Greater than 95% as determined by (a) Analysis by RP-HPLC; (b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
Molecular Weight : The molecular mass was determined and found to be 13203 Da by Mass Spectrum.
Amino-Acid Sequence : The full sequence was verified by MS/MS.
Endotoxin : Less than 0.08 ng/µg (0.8 IEU/µg) of rHuβ-NGF.
Form : The protein was lyophilized from a concentrated (1mg/ml) solution in 20mM PB, pH 7.2, 200mM NaCl buffer.
Reconstitution : It is recommended to reconstitute the lyophilized rHuβ-NGF in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Storage : Lyophilized rHuβ-NGF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution rHuβ-NGF should be stored at 4°C between 2-7 days and for future use below -18°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.
Gene Name : NGF nerve growth factor (beta polypeptide) [ Homo sapiens ]
Official Symbol : NGF
Synonyms : NGF; nerve growth factor (beta polypeptide); NGFB; HSAN5; Beta-NGF; MGC161428; Beta-nerve growth factor; nerve growth factor, beta subunit; nerve growth factor, beta polypeptide; beta-nerve growth factor; OTTHUMP00000013653; MGC161426
Gene ID : 4803
mRNA Refseq : NM_002506
Protein Refseq : NP_002497
MIM : 162030
UniProt ID : P01138
Chromosome Location : 1p13.1
Function : growth factor activity; rve growth factor receptor binding; receptor signaling protein activity

Online Inquiry


  •  
  • Note: There will be extra charge for optional service!
  • Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information

Optional Service: Optional requirements on this protein

Other Requirements:

Apply For A Coupon

$50 OFF Your First Purchase

Apply For a Coupon

Enter your email here to subscribe.

creative biomart inc.

Easy access to products and services you need from our library via powerful searching tools.

Follow Us

Copyright © 2007 – 2020 Creative BioMart. All Rights Reserved.