Recombinant Human NGF
Cat.No. : | NGF-495H |
Product Overview : | Recombinant Human Beta-NGF produced in CHO cells is a noncovalently disulfide-linked homodimer, of two 118 amino acids polypeptide monomers and having a molecular mass of 26.5 kDa. |
- Specification
- Gene Information
- Related Products
Cat. No. : | NGF-495H |
Description : | Beta-NGF is a neurotrophic factor structurally related to BDNF, NT-3 and NT-4. These proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures. Beta-NGF is a potent neurotrophic factor that signals through its receptor beta-NGFR, and plays a crucial role in the development and preservation of the sensory and sympathetic nervous systems. Beta-NGF also acts as a growth and differentiation factor for B lymphocytes and enhances B-cell survival. The functional form of human beta-NGF is a homodimer and the three disulfide bonds are required for biological activity. |
Biological Activity : | The ED50 as determined by its ability to stimulate chick E9 DRG neurite outgrowth was found to be < 1 ng/ml, corresponding to a Specific Activity of 1.0 x 10⁶ IU/mg. |
Purity : | Greater than 95% as determined by (a) Analysis by RP-HPLC; (b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. |
Molecular Weight : | The molecular mass was determined and found to be 13203 Da by Mass Spectrum. |
Amino-Acid Sequence : | The full sequence was verified by MS/MS. |
Endotoxin : | Less than 0.08 ng/µg (0.8 IEU/µg) of rHuβ-NGF. |
Form : | The protein was lyophilized from a concentrated (1mg/ml) solution in 20mM PB, pH 7.2, 200mM NaCl buffer. |
Reconstitution : | It is recommended to reconstitute the lyophilized rHuβ-NGF in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
Storage : | Lyophilized rHuβ-NGF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution rHuβ-NGF should be stored at 4°C between 2-7 days and for future use below -18°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles. |
Gene Name : | NGF nerve growth factor (beta polypeptide) [ Homo sapiens ] |
Official Symbol : | NGF |
Synonyms : | NGF; nerve growth factor (beta polypeptide); NGFB; HSAN5; Beta-NGF; MGC161428; Beta-nerve growth factor; nerve growth factor, beta subunit; nerve growth factor, beta polypeptide; beta-nerve growth factor; OTTHUMP00000013653; MGC161426 |
Gene ID : | 4803 |
mRNA Refseq : | NM_002506 |
Protein Refseq : | NP_002497 |
MIM : | 162030 |
UniProt ID : | P01138 |
Chromosome Location : | 1p13.1 |
Function : | growth factor activity; rve growth factor receptor binding; receptor signaling protein activity |
Products Types
◆ Recombinant Protein | ||
Ngf-1826R | Recombinant Rat Ngf Protein, His-tagged | +Inquiry |
NGF-1157C | Recombinant Cattle NGF Protein, His-tagged | +Inquiry |
Ngf-1828M | Recombinant Mouse Ngf Protein, His-tagged | +Inquiry |
NGF-2511H | Recombinant Human NGF protein(131-230 aa), N-SUMO & C-His-tagged | +Inquiry |
NGF-1154R | Recombinant Rhesus monkey NGF Protein, His-tagged | +Inquiry |
◆ Native Protein | ||
Ngf-298M | Active Native Mouse Nerve Growth Factor | +Inquiry |
Ngf-51M | Native Mouse Nerve Growth Factor 2.5S | +Inquiry |
Ngf-182M | Active Native Mouse Ngf Protein | +Inquiry |
◆ Lysates | ||
NGF-001MCL | Recombinant Mouse NGF cell lysate | +Inquiry |
NGF-001HCL | Recombinant Human NGF cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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