Active Recombinant Rat Gdnf protein, His-tagged

• Cat.No.: Gdnf-7431R
• Product Overview: Recombinant Rat Gdnf protein(Q07731-1)(Ser78-Ile211), fused with His tag, was expressed in Insect Cells.

Specification

• Source: Insect Cells
• Species: Rat
• Tag: His
• Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: 1. Measured in a cell proliferation assay using SH‑SY5Y human neuroblastoma cells. The ED50 for this effect is typically 10-50 ng/mL in the presence of Recombinant Rat GFR alpha‑1/GDNF R alpha‑1 His Chimera.2. Immobilized rat His-GDNF (78-211) at 10 μg/ml (100 μl/well) can bind rat GFRA1-Fc, The EC50 of rat GFRA1-Fc is 20-46.6 ng/ml.
• Molecular Mass: The recombinant rat GDNF consists of 150 amino acids and predicts a molecular mass of 17.1 KDa. It migrates as an approximately 20 KDa band in SDS-PAGE under reducing conditions.
• Protein Length: Ser78-Ile211
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Gdnf glial cell derived neurotrophic factor [ Rattus norvegicus ]
• Official Symbol: Gdnf
• Synonyms: GDNF; glial cell derived neurotrophic factor; glial cell line-derived neurotrophic factor; ATF; astrocyte-derived trophic factor; Glial cell line derived neutrophic factor; glial cell line derived neurotrophic factor
• Gene ID: 25453
• mRNA Refseq: NM_019139
• Protein Refseq: NP_062012