Native Human TNNI3

• Cat.No.: TNNI3-221H
• Product Overview: Native Human TNNI3 was purified from Human cardiac tissue by Tn-C affinity chromatography.

Specification

• Cat. No.: TNNI3-221H
• Description: Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM).
• Source: Human cardiac tissue
• Molecular Mass: 24 KDa
• Formulation: Liquid
• Storage buffer: 8 M Urea, 50 mM Tris.HCl, 15 mM ß-mercaptoethanol, 1 mM EDTA (pH 8.0)
• Applications: This peptide can be used with studies using ab7829, ab8290, ab8291 and ab137719.
• Biological activity: SDS PAGE profile of a typical recombinant Troponin-I preparation (3.8ug) showed a major band at 24kDa and a minor band attributable to Troponin-I dimer. Please note: Troponin-I is very susceptible to proteolytic breakdown within cardiac tissue and it is difficult to isolate undegraded native human cardiac troponin-I. This product is greater than 70% intact and hence proteolytic fragments can be observed.
• Storage: Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
• OfficialSymbol: TNNI3

Gene Information

• Gene Name: TNNI3 troponin I type 3 (cardiac) [ Homo sapiens ]
• Synonyms: TNNI3; troponin I type 3 (cardiac); CMH7; RCM1; cTnI; CMD2A; TNNC1; MGC116817; TNNI3; troponin I, cardiac; Troponin I, cardiac muscle; Cardiac troponin I; familial hypertrophic cardiomyopathy 7
• Gene ID: 7137
• mRNA Refseq: NM_000363
• Protein Refseq: NP_000354
• MIM: 191044
• UniProt ID: P19429
• Chromosome Location: 19q13.4
• Pathway: Hypertrophic cardiomyopathy (HCM); Muscle contraction; Cardiac muscle contraction
• Function: actin binding; calcium-dependent protein binding; protein domain specific binding; protein kinase binding; troponin C binding; troponin T binding; calcium channel inhibitor activity