Recombinant Human PRNP, His-tagged

• Cat.No.: PRNP-7569H
• Product Overview: Recombinant human PRNP protein, fused to His-tag at N-terminus, was expressed in E.coli.

Specification

• Antigen Description: Prion protein, also known as PRNP, is a ubiquitous membrane glycoprotein whose abnormal self-replicating, misfolded form is widely believed to cause several central nervous system disorders, collectively known as Transmissible Spongiform Encephalopathies (TSE). This protein contains a highly unstable region of five tandem octapeptide repeat. Mutations in the repeat region as well as elsewhere in this gene have been associated with Creutzfeldt-Jakob disease, fatal familial insomnia, Gerstmann-Straussler disease, Huntington disease-like 1, and kuru.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1M Urea, 10% glycerol
• Molecular Mass: 25 kDa (229aa)
• Protein length: 23-230aa
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MKKRPKPGGW NTGGSRYPGQ GSPGGNRYPP QGGGGWGQPH GGGWGQPHGG GWGQPHGGGW GQPHGGGWGQ GGGTHSQWNK PSKPKTNMKH MAGAAAAGAV VGGLGGYVLG SAMSRPIIHF GSDYEDRYYR ENMHRYPNQV YYRPMDEYSN QNNFVHDCVN ITIKQHTVTT TTKGENFTET DVKMMERVVE QMCITQYERE SQAYYQRGS
• Purity: >90% as determined by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/ml

Gene Information

• Gene Name: PRNP prion protein [ Homo sapiens ]
• Official Symbol: PRNP
• Synonyms: PRNP; prion protein; CJD, GSS, prion protein (p27 30) , PRIP; major prion protein; CD230; Creutzfeldt Jakob disease; fatal familial insomnia; Gerstmann Strausler Scheinker syndrome; p27 30; PRP; CD230 antigen; prion-related protein; CJD; GSS; PrP; ASCR; PRIP; PrPc; p27-30; PrP27-30; PrP33-35C; MGC26679
• Gene ID: 5621
• mRNA Refseq: NM_000311
• Protein Refseq: NP_000302
• MIM: 176640
• UniProt ID: P04156
• Chromosome Location: 20p13
• Pathway: Axon guidance, organism-specific biosystem; Developmental Biology, organism-specific biosystem; Glypican 1 network, organism-specific biosystem; NCAM signaling for neurite out-growth, organism-specific biosystem; NCAM1 interactions, organism-specific biosystem; Prion diseases, organism-specific biosystem; Prion diseases, conserved biosystem
• Function: ATP-dependent protein binding; chaperone binding; copper ion binding; copper ion binding; identical protein binding; metal ion binding; microtubule binding; protein binding; tubulin binding

Reviews

02/15/2020

PRNP showed good solubility in Western blot and could be easily dissolved in various buffers.

04/25/2019

The stability of PRNP was still very good under different environmental conditions, indicating its wide applicability and stability.

04/12/2019

Using PRNP for experiments, I can perform experimental manipulations more easily without having to worry about the reproducibility of experimental results.

Q&As

What is the role of PRNP in the treatment of neurodegenerative diseases?

Regulating the expression level of PRNP can influence the development of neurodegenerative diseases and the treatment effect. For example, certain drugs can inhibit the death of neurons and slow the progression of the disease by inhibiting the expression level of PRNP.

How does PRNP relate to other biomarkers?

PRNP has some association with other biomarkers. For example, in Creutzkok disease, abnormal folding and aggregation of PRNP proteins can be used with biomarkers such as amyloid to assess the diagnosis and prognosis of the disease.

Why is the expression level of RNP diagnostic and prognostic for neurodegenerative diseases?

PRNP expression levels can be used to aid in diagnosing neurodegenerative diseases and assessing prognosis. For example, in Alzheimer's disease, detecting mutations in the PRNP gene can predict disease development and prognosis.

How to use mutation or abnormal expression of PRNP for disease diagnosis and prognosis assessment?

It can be used to aid in diagnosing neurological diseases and assessing prognosis by detecting mutations and expression levels of the PRNP gene. For example, in Creutzkok disease, detecting mutations in the PRNP gene can predict the diagnosis and prognosis of the disease.

How are PRNP expression levels related to neurodegenerative diseases?

The expression level of PRNP is associated with the occurrence and progression of neurodegenerative diseases. For example, in Alzheimer's disease and Parkinson's disease, PRNP expression levels can change and may contribute to the development of the disease.

Which diseases are PRNP mutations or abnormalities associated with?

Mutations or abnormal expression of PRNP may be associated with a variety of neurological disorders. For example, in Creutzkok disease, mutations in the PRNP gene lead to abnormal protein folding and aggregation, which in turn causes the disease. In addition, in diseases such as Alzheimer's disease and Parkinson's disease, PRNP expression levels may also change and promote the development of the disease.