Recombinant Human ARG1 protein, His-tagged ARG1-3913H

Recombinant Human ARG1 protein, His-tagged

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Recombinant Human ARG1 protein, His-tagged

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Cat.No. : ARG1-3913H
Product Overview : Recombinant Human ARG1 (Met1-Lys322) protein was fused to His-tag at C-terminus and expressed in human 293 cells (HEK293).
Description : Arginase-1 (ARG1) is also known as Liver-type arginase, Type I arginase, which belongs to the arginase family. Arginase-1/ARG1 is a manganese-containing enzyme. The reaction catalyzed by this enzyme is: arginine + H2O → ornithine + urea. It is the final enzyme of the urea cycle. Defects in Arginase-1/ARG1 are the cause of argininemia (ARGIN).
Source : HEK293
Species : Human
Tag : His
Predicted N Terminal : Met1
Form : Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5, 10% trehalose.
Molecular Mass : The protein has a calculated MW of 35.6 kDa. The protein migrates as 35-38 kDa under reducing (R) condition (SDS-PAGE).
Protein length : Met1-Lys322
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE of ARG1-3913H :

Human Arginase 1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Gene Name : ARG1
Official Symbol : ARG1
Synonyms : ARG1; arginase, liver; arginase-1; type I arginase; liver-type arginase
Gene ID : 383
mRNA Refseq : NM_000045
Protein Refseq : NP_000036
MIM : 608313
UniProt ID : P05089

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