Active Recombinant Human ARG1 protein, His-tagged

• Cat.No.: ARG1-3913H
• Product Overview: Active Recombinant Human ARG1 protein(P05089-1) (Met 1-Lys 322) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 1-322 aa
• Form: Lyophilized from sterile 20mM Tris, 150mM NaCl, 20% Glycerol, 1mM DTT, pH 7.4.
• Bio-activity: Measured by the production of urea during the hydrolysis of arginine. The specific activity is >35,000 pmoles/min/μg.
• Molecular Mass: The secreted recombinant human ARG1 consists of 333 amino acids and has a calculated molecular mass of 36.2 kDa. The apparent molecular mass of the protein is approximately 40 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: ARG1 arginase, liver [ Homo sapiens ]
• Official Symbol: ARG1
• Synonyms: ARG1; arginase, liver; arginase-1; type I arginase; liver-type arginase
• Gene ID: 383
• mRNA Refseq: NM_000045
• Protein Refseq: NP_000036
• MIM: 608313
• UniProt ID: P05089