Recombinant Human ARG1 protein, His-tagged

• Cat.No.: ARG1-3913H
• Product Overview: Recombinant Human ARG1 (Met1-Lys322) protein was fused to His-tag at C-terminus and expressed in human 293 cells (HEK293).

Specification

• Description: Arginase-1 (ARG1) is also known as Liver-type arginase, Type I arginase, which belongs to the arginase family. Arginase-1/ARG1 is a manganese-containing enzyme. The reaction catalyzed by this enzyme is: arginine + H2O → ornithine + urea. It is the final enzyme of the urea cycle. Defects in Arginase-1/ARG1 are the cause of argininemia (ARGIN).
• Source: HEK293
• Species: Human
• Tag: His
• Predicted N Terminal: Met1
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5, 10% trehalose.
• Molecular Mass: The protein has a calculated MW of 35.6 kDa. The protein migrates as 35-38 kDa under reducing (R) condition (SDS-PAGE).
• Protein length: Met1-Lys322
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: ARG1
• Official Symbol: ARG1
• Synonyms: ARG1; arginase, liver; arginase-1; type I arginase; liver-type arginase
• Gene ID: 383
• mRNA Refseq: NM_000045
• Protein Refseq: NP_000036
• MIM: 608313
• UniProt ID: P05089