Active Human BMP2

• Cat.No.: BMP2-30H
• Product Overview: A DNA sequence encoding the mature form of human BMP2 ( NP_001191.1 ) ( Gln283 - Arg396 ) was expressed.

Specification

• Species: Human
• Source: Human
• Tag: Non
• Protein Length: 283-396 a.a.
• Description: BMP2 is a member of the BMP subgroup belonging to the transforming growth factor superfamily. The mature BMP2 acts as disulfide-linked homodimers and heterodimers with BMP7 after proteolytic removal of propeptide. BMP2 is capable of inducing the formation of cartilage and bone but are now regarded as multifunctional cytokines.
• Form: Supplied as a 0.2 μm filtered solution of 40% acetonitrile + 0.1% TFA.
• Purity: > 98 % as determined by SDS-PAGE
• Bio-activity: Measured by its ability to induce alkaline phosphatase production by MC3T3-E1 mouse osteoblastic cells. The ED50 for this effect is typically 0.5 - 0.9 μg / ml.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70°C.
• Predicted N terminal: Met
• Molecular Mass: The recombinant human BMP2 monomer consists of 115 amino acids and has a predicted molecular mass of 13 kDa. It migrates with the apparent molecular mass of 13 & 26 kDa corresponding to the monomer & homodimer in SDS-PAGE under reducing conditions.
• Storage: Store it under sterile conditions at -70°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Pathways: Adipogenesis; Basal cell carcinoma; Cytokine-cytokine receptor interaction; Heart Development; Hedgehog signaling pathway; Id Signaling Pathway; Pathways in cancer; Senescence and Autophagy; Signaling by BMP; TGF-beta signaling pathway

Gene Information

• Gene Name: BMP2 bone morphogenetic protein 2 [ Homo sapiens ]
• Official Symbol: BMP2
• Synonyms: BMP2; bone morphogenetic protein 2; BMP2A; BMP-2A; OTTHUMP00000030228; bone morphogenetic protein 2A; Bone morphogenetic protein 2; Bone morphogenetic protein 2A
• Gene ID: 650
• mRNA Refseq: NM_001200
• Protein Refseq: NP_001191
• MIM: 112261
• UniProt ID: P12643
• Chromosome Location: 20p12
• Function: receptor activity; protein binding; BMP receptor binding; activin receptor activity; growth factor activity; SMAD binding; cytokine activity; phosphatase activator activity; protein domain specific binding; protein heterodimerization activity; transforming growth factor beta receptor binding; transmembrane receptor protein serine/threonine kinase activity

Citation

Protein engineering of recombinant human bone morphogenetic protein 2 with higher interaction with Ca phosphate based scaffold used for osteogenesis.

Journal: Journal of biomedical materials research. Part A    PubMed ID: 28643418    Data: 2018/5/7

Authors: Mohammad Bayat, Sahar Shojaei, Naeim Bahrami

Article Snippet:Sharapova N.E and his colleagues expressed this protein in E.Coli BL21 (DE3) and approved biological activity with in vitro induction of alkaline phosphatase synthesis in C2C12 and C3H10T1/2 cell culture and in vivo osteogenesis in site of injection.. Nowadays GMP Recombinant Human Bone Morphogenetic Protein 2(BMP2-123H) protein ref seq: NP001191 is produced by E.Coli expression system.Sharapova N.E and his colleagues expressed this protein in E.Coli BL21 (DE3) and approved biological activity with in vitro induction of alkaline phosphatase synthesis in C2C12 and C3H10T1/2 cell culture and in vivo osteogenesis in site of injection.. Nowadays GMP Recombinant Human Bone Morphogenetic Protein 2(BMP2-123H) protein ref seq: NP001191 is produced by E.Coli expression system.. This protein is expressed with sequence (Gln 283-Arg 396) of Human BMP2 by Creative BioMart Company. . Its Bioactivity is less than 50 ng/ml.Its Bioactivity is less than 50 ng/ml.