"CD40LG" Related Products

Recombinant Human CD40LG Protein, Fc-Avi-tagged, Biotinylated

Cat.No. : CD40LG-280H
Product Overview : Biotinylated Human CD40 Ligand, Fc Tag is expressed from HEK293 cells. It contains AA Met 113 - Leu 261 (Accession # P29965-1). This protein carries an Avi tag at the N-terminus, followed by a human IgG1 Fc tag.
Source : HEK293
Species : Human
Tag : Fc-Avi
Predicted N Terminal : Gly
Form : Lyophilized from 0.22 um filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5, 10% trehalose.
Bio-activity : Immobilized Human CD40, His Tag at 2 ug/mL (100 uL/well) can bind Biotinylated Human CD40 Ligand, Fc Tag with a linear range of 1-31 ug/mL.
Molecular Mass : The protein has a calculated MW of 45.0 kDa. The protein migrates as 50-55 kDa under reducing condition (SDS-PAGE) due to glycosylation.
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Characteristic : Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin. The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
Stability : Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage : For long term storage, the product should be stored at lyophilized state at -20ºC or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8ºC for 12 months in lyophilized state; -70ºC for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name : CD40LG CD40 ligand [ Homo sapiens ]
Official Symbol : CD40LG
Synonyms : CD40LG; CD40 ligand; HIGM1, IMD3, TNFSF5, tumor necrosis factor (ligand) superfamily, member 5 (hyper IgM syndrome); CD40 antigen ligand; CD40L; CD154; gp39; hCD40L; hyper IgM syndrome; T B cell activating molecule; TNF related activation protein; TRAP; tumor necrosis factor (ligand) superfamily member 5; CD40-L; T-cell antigen Gp39; T-B cell-activating molecule; TNF-related activation protein; IGM; IMD3; HIGM1; T-BAM; TNFSF5;
Gene ID : 959
mRNA Refseq : NM_000074
Protein Refseq : NP_000065
MIM : 300386
UniProt ID : P29965

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