Active Recombinant Rat Cd86 protein, His-tagged

• Cat.No.: Cd86-4087R
• Product Overview: Recombinant Rat Cd86 protein(Val 29 - Ile 250), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Rat
• Tag: His
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with Trehalose as protectant.
• Bio-activity: Immobilized Rat CTLA-4, Fc Tag at 5 μg/mL (100 μL/well) can bind Rat B7-2, His Tag with a linear range of 0.02-1.25 μg/mL.Immobilized Human CTLA-4, Fc Tag at 5 μg/mL (100 μL/well) can bind Rat B7-2, His Tag with a linear range of 0.01-0.625 μg/mL.Immobilized Mouse CTLA-4, Fc Tag at 5 μg/mL (100 μL/well) can bind Rat B7-2, His Tag with a linear range of 0.039-1.25 μg/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminusThe protein has a calculated MW of 27.3 kDa. The protein migrates as 40-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein Length: Val 29 - Ile 250
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Cd86 CD86 molecule [ Rattus norvegicus ]
• Official Symbol: Cd86
• Synonyms: CD86; CD86 molecule; T-lymphocyte activation antigen CD86; cd86 antigen; membrane glycoprotein; B7-2
• Gene ID: 56822
• mRNA Refseq: NM_020081
• Protein Refseq: NP_064466