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Recombinant Human Euchromatic Histone-lysine N-methyltransferase 1

Cat. No. : EHMT1-293H
Product Overview : Glucagon Like Peptide-1 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 30 amino acids and having a molecular mass of 3298.7 Dalton. The GLP-1 is purified by proprietary chromatographic techniques.
Description : Glucagon-like peptide-1 (GLP-1) is derived from the transcription product of the proglucagon gene. The major source of GLP-1 in the body is the intestinal L cell that secretes GLP-1 as a guthormone. The biologically active forms of GLP-1 are: GLP-1-(7-37) and GLP-1-(7-36)NH2. GLP-1 secretion by L cells is dependent on the presence of nutrients in the lumen of the small intestine. The secretagogues (agents that causes or stimulates secretion) of this hormone include major nutrients like carbohydrate, proteinand lipid.
Source : Human
Host : Escherichia Coli.
Form : The Recombinant GLP-1 concentrated solution (1 mg/ml) was lyophilized after extensive dialyses against 0.1 mg sodium phosphate monobasic & 1.6 mg sodium phosphate dibasic.
Purity : Greater than 95.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Bio-activity : 1. Regulates Glucose levels rapidly 2. Reduces Insulin resistence 3. Reduces Glucagon 4. Reduces HbA1c 5. Stimulates beta cell growth which stimulates insulin production.
Physical Appearance : Sterile Filtered White lyophilized (freeze-dried) powder.
Solubility : It is recommended to reconstitute the lyophilized Glucagon Like Peptide-1 in sterile 18Mμ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Amino acid sequence : The sequence of the first five N-terminal amino acids was determined and was found to be His-Ala-Glu-Gly-Thr.
Storage : Lyophilized Glucagon Like Peptide-1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution GLP-1 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Pathways : Lysine degradation
Gene Name : EHMT1 euchromatic histone-lysine N-methyltransferase 1 [ Homo sapiens ]
Official Symbol : EHMT1
Synonyms : EHMT1; euchromatic histone-lysine N-methyltransferase 1; GLP; GLP1; KMT1D; FP13812; FLJ12879; KIAA1876; EUHMTASE1; Eu-HMTase1; bA188C12.1; DKFZp667M072; RP11-188C12.1; histone-lysine N-methyltransferase EHMT1; H3-K9-HMTase 5; G9a like protein; G9a-like protein 1; OTTHUMP00000022711; OTTHUMP00000022713; OTTHUMP00000212228; lysine N-methyltransferase 1D; histone H3-K9 methyltransferase 5; histone-lysine N-methyltransferase, H3 lysine-9 specific 5; NP_001138999.1; EC 2.1.1.43; NP_079033.4
Gene ID : 79813
mRNA Refseq : NM_001145527
Protein Refseq : NP_001138999
MIM : 607001
UniProt ID : Q9H9B1
Chromosome Location : 9q34.3
Function : histone methyltransferase activity (H3-K27 specific); histone methyltransferase activity (H3-K9 specific); histone-lysine N-methyltransferase activity; metal ion binding; methyltransferase activity; p53 binding; protein-lysine N-methyltransferase activity; transferase activity; zinc ion binding

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