Recombinant Human Interleukin 20 IL20-500H

Recombinant Human Interleukin 20

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Recombinant Human Interleukin 20

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Cat. No. : IL20-500H
Product Overview : Recombinant Human IL-20 produced in E.Coli is a homodimeric, non-glycosylated polypeptide chain containing 2 x 153 amino acids and having a total molecular mass of 35,212 Dalton.
Description : The newly discovered cytokine interleukin-20 (IL-20) is structurally related to IL-10, yet it appears to be an autocrine factor for keratinocytes that regulates their participation in inflammation.
Species : Human
Source : E.coli
Form : Recombinant hIL-20 was lyophilized from a concentrated (1mg/ml) solution containing 100mM sodium chloride and 10mM sodium citrate buffer pH-3.
Purity : Purity Greater than 98% as determined by: Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE.
Bio-activity : Human IL-20 is fully biologically active when compared to standard. The Biological Acitivity was determined by the ability to activiate STAT following receptor ligand interaction.
Physical Appearance : Sterile Filtered white lyophilized (freeze-dried) powder.
Solubility : Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg"s/ml, which can be further diluted into other aqueous solutions.
Purity Greater than 98% as determined by : Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE.
Protein Content determined by : UV spectroscopy at 280 nm. Analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.
Endotoxin Level : Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
Amino acid sequence : MLKTLNLGSC VIATNLQEIR NGFSEIRGSV QAKDGNIDIR ILRRTESLQD TKPANRCCLL RHLLRLYLDR VFKNYQTPDH YTLRKISSLA NSFLTIKKDL RLCHAHMTCH CGEEAMKKYS QILSHFEKLE PQAAVVKALG ELDILLQWME ETE
Storage : Lyophilized product is very stable at -20 degrees. Reconstituted material should be aliquoted and frozen at -20. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.
Pathways : Cytokine-cytokine receptor interaction; Jak-STAT signaling pathway
Gene Name : IL20 interleukin 20 [ Homo sapiens ]
Official Symbol : IL20
Synonyms : IL20; interleukin 20; IL-20; IL10D; ZCYTO10; MGC96907; OTTHUMP00000034618; four alpha helix cytokine; OTTHUMP00000034617; Four alpha helix cytokine Zcyto10
Gene ID : 50604
mRNA Refseq : NM_018724
Protein Refseq : NP_061194
MIM : 605619
UniProt ID : Q9NYY1
Chromosome Location : 1q32
Function : cytokine activity; interleukin-20 receptor binding

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