||Recombinant human MAPK13 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
||MAPK13, also known as mitogen-activated protein kinase 13, is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. It is a related protein that is phosphorylated by MKK6 in response to cytokines and cellular stresses.
||Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol 0.1M NaCl,1mM DTT
||44.2 kDa (385aa), confirmed by MALDI-TOF
||> 90% by SDS - PAGE
||1.0 mg/ml (determined by Bradford assay)
|Sequences of amino acids:
||MGSSHHHHHH SSGLVPRGSH MSLIRKKGFY KQDVNKTAWE LPKTYVSPTH VGSGAYGSVC SAIDKRSGEK VAIKKLSRPF QSEIFAKRAY RELLLLKHMQ HENVIGLLDV FTPASSLRNF YDFYLVMPFM QTDLQKIMGM EFSEEKIQYL VYQMLKGLKY IHSAGVVHRD LKPGNLAVNE DCELKILDFG LARHADAEMT GYVVTRWYRA PEVILSWMHY NQTVDIWSVG CIMAEMLTGK TLFKGKDYLD QLTQILKVTG VPGTEFVQKL NDKAAKSYIQ SLPQTPRKDF TQLFPRASPQ AADLLEKMLE LDVDKRLTAA QALTHPFFEP FRDPEEETEA QQPFDDSLEH EKLTVDEWKQ HIYKEIVNFS PIARKDSRRR SGMKL
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
||Amyotrophic lateral sclerosis (ALS); Epithelial cell signaling in Helicobacter pylori infection; Fc epsilon RI signaling pathway; GnRH signaling pathway; Leukocyte transendothelial migration; MAPK signaling pathway; Neurotrophin signaling pathway; RIG-I-like receptor signaling pathway; T cell receptor signaling pathway; Toll-like receptor signaling pathway; VEGF signaling pathway; Signalling by NGF