Recombinant Human, HSPE1 His-tagged

• Cat.No.: HSPE1-3679H
• Product Overview: Total 111 AA. MW: 12.0 kDa (calculated). N-Terminal His-tag (10 extra AA)

Specification

• Cat. No.: HSPE1-3679H
• Description: Heat shock 10kDa protein 1 (chaperonin 10) , also known as HSPE1, GroES or cpn10 , is a chaperonin which usually works in conjunction with GroEL.[1] In eukaryotes, it is very similar to Heat Shock Protein 10 or Hsp10.
• Source: E. coli
• Species: Human
• Form: Filtered (0,4 μm) and lyophilized in 0.5 mg/mL in 0.05 M phosphate buffer, 0.075 M NaCl, pH 7.4
• Purity: >90%
• Reconstitution: Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
• Applications: ELISA; Western blotting
• Quality Control Test: BCA to determine quantity of the protein; SDS PAGE to determine purity of the protein; LAL to determine quantity of endotoxin
• Sequences of amino acids: MKHHHHHHAS AGQAFRKFLP LFDRVLVERS AAETVTKGGI MLPEKSQGKV LQATVVAVGS GSKGKGGEIQ PVSVKVGDKV LLPEYGGTKV VLDDKDYFLF RDGDILGKYV D
• Storage: Store lyophilized protein at -80°C. Lyophilized protein remains stable until the expiry date when stored at -80°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at -80°C for long term storage. Reconstituted protein can be stored at 4°C for a week.

Gene Information

• Gene Name: HSPE1 heat shock 10kDa protein 1 (chaperonin 10) [ Homo sapiens ]
• Official Symbol: HSPE1
• Synonyms: CPN10; GROES; HSP10; EPF; HSP10; Hsp10; 10 kDa chaperonin; 10CPN10; GROES; HSP10; EPF; HSP10; Hsp10; kDa heat shock protein; mitochondrial; Early-pregnancy factor; chaperonin10;heat shock 10kD protein 1; HSPE1; heat shock 10kDa protein 1 (chaperonin 10)10 kDa chaperonin; 10 kDa heat shock protein; mitochondrial; Early-pregnancy factor; chaperonin 10; heat shock 10kD protein 1
• Gene ID: 3336
• mRNA Refseq: NM_002157
• Protein Refseq: NP_002148
• MIM: 600141
• UniProt ID: P61640
• Chromosome Location: 2q33.1
• Pathway: ATP binding, chaperone binding, unfold protein binding
• Function: caspase activation, protein folding, response to unfolded protein