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Recombinant Human, HSPA5 His-tagged

Cat.No. : HSPA5-3677H
Product Overview : Total 646 AA. MW: 71.72 kDa (calculated). N-Terminal His-tag, 10 extra AA (highlighted).
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Cat. No. : HSPA5-3677H
Description : Binding immunoglobulin protein (BiP) also known as 78 kDa glucose-regulated protein (GRP-78) or heat shock 70 kDa protein 5 (HSPA5) is a HSP70 molecular chaperone located in the lumen of the endoplasmic reticulum (ER), that binds newly-synthesized proteins as they are translocated into the ER and maintains them in a state competent for subsequent folding and oligomerization. BiP is also an essential component of the translocation machinery, as well as playing a role in retrograde transport across the ER membrane of aberrant proteins destined for degradation by the proteasome. BiP is an abundant protein under all growth conditions, but its synthesis is markedly induced under conditions that lead to the accumulation of unfolded polypeptides in the ER. In humans, BiP is encoded by the HSPA5 gene.
Source : E. coli
Species : Human
Form : Filtered (0,4 μm) and lyophilized in 0.5 mg/mL in 20mM TRIS, 50mM NaCl, pH 7.5
Reconstitution : Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Applications : Western blotting
Quality Control Test : BCA to determine quantity of the protein; SDS PAGE to determine purity of the protein.
Sequences of amino acids : MKHHHHHHAS EEEDKKEDVG TVVGIDLGTT YSCVGVFKNG RVEIIANDQG NRITPSYVAF TPEGERLIGD AAKNQLTSNP ENTVFDAKRL IGRTWNDPSV QQDIKFLPFK VVEKKTKPYI QVDIGGGQTK TFAPEEISAM VLTKMKETAE AYLGKKVTHA VVTVPAYFND AQRQATKDAG TIAGLNVMRI INEPTAAAIA YGLDKREGEK NILVFDLGGG TFDVSLLTID NGVFEVVATN GDTHLGGEDF DQRVMEHFIK LYKKKTGKDV RKDNRAVQKL RREVEKAKRA LSSQHQARIE IESFYEGEDF SETLTRAKFE ELNMDLFRST MKPVQKVLED SDLKKSDIDE IVLVGGSTRI PKIQQLVKEF FNGKEPSRGI NPDEAVAYGA AVQAGVLSGD QDTGDLVLLD VCPLTLGIET VGGVMTKLIP RNTVVPTKKS QIFSTASDNQ PTVTIKVYEG ERPLTKDNHL LGTFDLTGIP PAPRGVPQIE VTFEIDVNGI LRVTAEDKGT GNKNKITITN DQNRLTPEEI ERMVNDAEKF AEEDKKLKER IDTRNELESY AYSLKNQIGD KEKLGGKLSS EDKETMEKAV EEKIEWLESH QDADIEDFKA KKKELEEIVQ PIISKLYGSA GPPPTGEEDT AEKDEL
Storage : Store lyophilized protein at -20°C. Lyophilized protein remains stable until the expiry date when stored at -20°C. Aliquo treconstituted protein to avoid repeated freezing/thawing cycles and store at -80°C for long term storage. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after one week at 4°C.
Gene Name : HSPA5 heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa) [ Homo sapiens ]
Official Symbol : HSPA5
Synonyms : HSPA5; heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa); HSPA5 heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa); BIP; MIF2; GRP78; GRP 78; FLJ26106; BiP; OTTHUMP00000022124; Heat shock 70 kDa protein 5; Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; Heat-shock 70kD protein-5 (glucose-regulated protein, 78kD); Immunoglobulin heavy chain-binding protein; heat shock 70kD protein 5 (glucose-regulated protein, 78kD); heat shock 70kDa protein 5
Gene ID : 3309
mRNA Refseq : NM_005347
Protein Refseq : NP_005338
MIM : 138120
UniProt ID : P11021
Chromosome Location : 9q33-q34.1
Pathway : Antigen processing and presentation; Neurodegenerative Disorders; Prion disease; Hemostasis.
Function : ATP binding; calcium ion binding; caspase inhibitor activity; nucleotide binding; protein binding, bridging; ribosome binding; unfolded protein binding.

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (5)

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Are there drugs targeting HSPA5 currently in development? 03/16/2021

Some studies explore small molecules and compounds that target HSPA5 as potential therapeutic agents, but clinical applications are still under investigation.

In what other medical conditions is HSPA5 relevant? 01/27/2021

HSPA5 is involved in various conditions, including cardiovascular diseases, diabetes, and neurodegenerative disorders.

Are there diagnostic applications of HSPA5 in cancer? 03/16/2018

Elevated levels of HSPA5 in certain cancers may serve as a diagnostic marker, providing information about the cellular stress response and potential malignancy.

Is there a relationship between HSPA5 and autoimmune disorders? 11/19/2017

HSPA5's involvement in the immune response suggests potential links to autoimmune disorders, but specific mechanisms are still being studied.

How does HSPA5 influence the immune response? 03/25/2016

HSPA5 is involved in antigen presentation, linking its role in protein folding to the modulation of immune responses.

Customer Reviews (3)

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Reviews
07/02/2022

    This level of support ensures that researchers have a seamless experience, enabling them to focus on their research goals without worrying about logistical or technical concerns.

    01/18/2020

      Its versatility in various biological applications, along with its compatibility with different techniques, strongly recommend it as a preferred reagent for researchers in the field.

      07/07/2019

        researchers highly appreciate HSPA5 protein for its excellent performance in ELISA assays and protein electron microscopy structure analysis.

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