Recombinant Human CSF1 (33-190aa), Met tagged CSF1-033E

Recombinant Human CSF1 (33-190aa), Met tagged

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Recombinant Human CSF1 (33-190aa), Met tagged

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Cat. No. : CSF1-033E
Product Overview : Recombinant Human CSF1 (Glu33-Ser190, with an N­terminal Met) was expressed in E. coli and produced using non­animal reagent in an animal­free laboratory.
Description : M­CSF, also known as CSF­1, is a four-­α­-helical bundle cytokine that is the primary regulator of macrophage survival, proliferation and differentiation. M­CSF is also essential for the survival and proliferation of osteoclast progenitors. M­CSF also primes and enhances macrophage killing of tumor cells and microorganisms, regulates the release of cytokines and other inflammatory modulators from macrophages, and stimulates pinocytosis . M­CSF increases during pregnancy to support implantation and growth of the decidua and placenta. Sources of M­CSF include fibroblasts, activated macrophages, endometrial secretory epithelium, bone marrow stromal cells and activated endothelial cells.
Source : E. coli
N­terminal Sequence Analysi : Met
Molecular Mass : 18.5 kDa
SDS­PAGE : 37 kDa, non­reducing conditions
Activity : Measured in a cell proliferation assay using M­NFS­60 mouse myeloid cells. The ED50 for this effect is typically 0.5 ­ 1.5 ng/mL.
Endotoxin Leve : <1.0 EU per 1μg of the protein by the LAL method.
Purity : >97%, by SDS­PAGE under reducing conditions and visualized by silver stain.
Formulation : Lyophilized from a 0.2 μm filtered solution in PBS. See Certificate of Analysis for details.
Reconstitution : Reconstitute at 200 μg/mL in sterile PBS.
Shipping : The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage : Use a manual defrost freezer and avoid repeated freeze­thaw cycles. 1. 12 months from date of receipt, ­20 to ­70 °C as supplied. 2. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3. 3 months, ­20 to ­70 °C under sterile conditions after reconstitution.
Unitprot ID : P09603
PDB rendering basedon 1hmc. :

Gene Name : CSF1 colony stimulating factor 1 (macrophage) [ Homo sapiens ]
Official Symbol : CSF1
Synonyms : CSF1; colony stimulating factor 1; MCSF; MGC31930; macrophage colony-stimulating factor 1; CSF-1; lanimostim; macrophage colony stimulating factor
Gene ID : 1435
mRNA Refseq : NM_000757
Protein Refseq : NP_000748
MIM : 120420
Chromosome Location : 1p21-p13
Pathway : Cytokine-cytokine receptor interaction; Hematopoietic cell lineage; Osteoclast differentiation; Rheumatoid arthritis; Signaling events mediated by PTP1B; Signaling events mediated by TCPTP
Function : cytokine activity; growth factor activity; macrophage colony-stimulating factor receptor binding; protein homodimerization activity

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