| Cat. No. : |
CSF3-01H |
| Description : |
Granulocyte colony-stimulating factor (G-CSF or GCSF) is a colony-stimulating factor hormone. It is a glycoprotein, growth factor or cytokine produced by a number of different tissues to stimulate the bone marrow to produce granulocytes and stem cells. G-CSF then stimulates the bone marrow to release them into the blood. It also stimulates the survival, proliferation, differentiation, and function of neutrophil precursors and mature neutrophils. G-CSF is also known as colony-stimulating factor 3 (CSF 3). |
| Purity : |
>95% by SDS-PAGE and SEC-HPLC. |
| Formulation : |
Lyophilized after extensive dialysis against 10mM sodium acetate buffer pH=4. |
| Specific Activity : |
The ED50, calculated by the dose-dependant proliferation of murine NFS-60 indicator cells is less than 0.1 ng/ml, corresponding to a Specific Activity of 6.0x 107IU/ mg. |
| Endotoxin : |
Less than 0.03ng/µg (0.03IEU/µg) determined by LAL test. |
| Reconstitutionon : |
It is recommended to reconstitute the lyophilized rHuG-CSF in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Storagee : |
Lyophilized rHuG-CSF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution rHuG-CSF should be stored at 4°C between 2-7 days and for future use below -18°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Aliquot to avoid repeated freeze-thaw cycles. |
