Recombinant Human Granulocyte Colony Stimulating Factor

• Cat.No.: CSF3-01H
• Product Overview: Recombinant Human G-CSF produced inE. coliis a single, non-glycosylated, polypeptide chain containing 175 amino acids and having a molecular mass of 18.8 kDa.

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Description: Granulocyte colony-stimulating factor (G-CSF or GCSF) is a colony-stimulating factor hormone. It is a glycoprotein, growth factor or cytokine produced by a number of different tissues to stimulate the bone marrow to produce granulocytes and stem cells. G-CSF then stimulates the bone marrow to release them into the blood. It also stimulates the survival, proliferation, differentiation, and function of neutrophil precursors and mature neutrophils. G-CSF is also known as colony-stimulating factor 3 (CSF 3).
• Purity: >95% by SDS-PAGE and SEC-HPLC.
• Formulation: Lyophilized after extensive dialysis against 10mM sodium acetate buffer pH=4.
• Specific Activity: The ED50, calculated by the dose-dependant proliferation of murine NFS-60 indicator cells is less than 0.1 ng/ml, corresponding to a Specific Activity of 6.0x 10⁷IU/ mg.
• Endotoxin: Less than 0.03ng/µg (0.03IEU/µg) determined by LAL test.
• Reconstitutionon: It is recommended to reconstitute the lyophilized rHuG-CSF in sterile 18MΩ-cm H₂O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
• Storagee: Lyophilized rHuG-CSF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution rHuG-CSF should be stored at 4°C between 2-7 days and for future use below -18°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Aliquot to avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: CSF3
• Synonyms: G-CSF; GCSF; MGC45931; colony stimulating factor 3 (granulocyte); colony stimulating factor 3; granulocyte colony stimulating factor; pluripoietin; filgrastim; lenograstim; Granulocyte colony-stimulating factor precursor; CSF3
• Gene ID: 1440
• mRNA Refseq: NM_172219.1
• Protein Refseq: NP_757373.1
• MIM: 138970
• UniProt ID: P09919
• Chromosome Location: 17q11.2-q12
• Pathway: Cytokine-cytokine receptor interaction, Hematopoietic cell lineage, Jak-STAT signaling pathway
• Function: Cytokine activity, Enzyme binding, Granulocyte colony-stimulating factor receptor binding, Interleukin-6 receptor binding