Recombinant Human Granulocyte Colony Stimulating Factor CSF3-01H

Recombinant Human Granulocyte Colony Stimulating Factor

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Recombinant Human Granulocyte Colony Stimulating Factor

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Cat. No. : CSF3-01H
Product Overview : Recombinant Human G-CSF produced inE. coliis a single, non-glycosylated, polypeptide chain containing 175 amino acids and having a molecular mass of 18.8 kDa.
Description : Granulocyte colony-stimulating factor (G-CSF or GCSF) is a colony-stimulating factor hormone. It is a glycoprotein, growth factor or cytokine produced by a number of different tissues to stimulate the bone marrow to produce granulocytes and stem cells. G-CSF then stimulates the bone marrow to release them into the blood. It also stimulates the survival, proliferation, differentiation, and function of neutrophil precursors and mature neutrophils. G-CSF is also known as colony-stimulating factor 3 (CSF 3).
Purity : >95% by SDS-PAGE and SEC-HPLC.
Formulation : Lyophilized after extensive dialysis against 10mM sodium acetate buffer pH=4.
Specific Activity : The ED50, calculated by the dose-dependant proliferation of murine NFS-60 indicator cells is less than 0.1 ng/ml, corresponding to a Specific Activity of 6.0x 107IU/ mg.
Endotoxin : Less than 0.03ng/µg (0.03IEU/µg) determined by LAL test.
Reconstitutionon : It is recommended to reconstitute the lyophilized rHuG-CSF in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Storagee : Lyophilized rHuG-CSF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution rHuG-CSF should be stored at 4°C between 2-7 days and for future use below -18°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Aliquot to avoid repeated freeze-thaw cycles.
Crystal structure of 3 molecules of human G-CSF. FromPDB1rhg :

Gene Name : CSF3
Synonyms : G-CSF; GCSF; MGC45931; colony stimulating factor 3 (granulocyte); colony stimulating factor 3; granulocyte colony stimulating factor; pluripoietin; filgrastim; lenograstim; Granulocyte colony-stimulating factor precursor; CSF3
Gene ID : 1440
mRNA Refseq : NM_172219.1
Protein Refseq : NP_757373.1
MIM : 138970
UniProt ID : P09919
Chromosome Location : 17q11.2-q12
Pathway : Cytokine-cytokine receptor interaction, Hematopoietic cell lineage, Jak-STAT signaling pathway
Function : Cytokine activity, Enzyme binding, Granulocyte colony-stimulating factor receptor binding, Interleukin-6 receptor binding

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