Recombinant Human Sirtuin 5, His-tagged

• Cat.No.: SIRT5-4880H
• Product Overview: Recombinant full length human SIRT5 was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag.

Specification

• Cat. No.: SIRT5-4880H
• Description: SIRT5 is a member of the sirtuin family of proteins which are homologs to the yeast Sir2 protein. Sirtuin family contain a sirtuin core domain and are grouped into four classes with SIRT5 being a member of class III. SIRT5 consists of eight exons and is found in two isoforms which encode a 310 aa and a 299 aa protein, respectively. Human SIRT5 is most predominantly expressed in heart muscle cells and in lymphoblasts.
• Applications: SIRT Assay
• Molecular Weight: 39 kDa
• Expression System: Sf9 insect cells using baculovirus
• Species: Human
• Form: Recombinant protein stored in 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25 % glycerol.
• Specific Activity: 20 RLU/min/mg
• Purity: > 80 %
• Concentration: 0.1 ug/ul
• Sequences: Full-length
• Storage: Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Pathways: Signaling events mediated by HDAC Class I

Gene Information

• Gene Name: SIRT5 sirtuin 5 [ Homo sapiens ]
• Official Symbol: SIRT5
• Synonyms: SIRT5; sirtuin 5; SIR2L5; FLJ36950; NAD-dependent deacetylase sirtuin-5; sir2-like 5; sirtuin type 5; OTTHUMP00000016054; OTTHUMP00000016055; SIR2-like protein 5; silent mating type information regulation 2, S.cerevisiae, homolog 5; EC 3.5.1.-; sirtuin (silent mating type information regulation 2 homolog) 5 (S. cerevisiae); sirtuin (silent mating type information regulation 2, S. cerevisiae, homolog) 5
• Gene ID: 23408
• mRNA Refseq: NM_012241
• Protein Refseq: NP_036373
• MIM: 604483
• UniProt ID: Q9NXA8
• Chromosome Location: 6p23
• Function: NAD binding; NAD+ ADP-ribosyltransferase activity; hydrolase activity; hydrolase activity; metal ion binding; zinc ion binding