Cat. No. : |
TF-4777H |
Product Overview : |
Product is the lyophilized powder of human TF and buffer salts. Human TF is purified from pooled human serum using multi-step procedures which may include salt fractionation, gel filtration, ion-exchange chromatography and immunoabsorption. The product is dialyzed into 0.01M sodium phosphate, 0.07M sodium chloride, pH 7.3, filtered through a 0.22 µm filter, vialed and lyophilized. No preservative has been added. |
Description : |
TF is a glycoprotein that binds iron very tightly but reversibly. Although iron bound to transferrin is less than 0.1% (4 mg) of the total body iron, it is the most important iron pool, with the highest rate of turnover (25 mg/24 h). TF has a molecular weight of around 80 kDa and contains 2 specific high-affinity Fe (III) binding sites. |
Source : |
Human Plasma |
Species : |
Human |
Preparation : |
The protein is purified from pooled human plasma and lyophilized from 0.02M NH4HCO3. |
Reconstitution : |
The protein is readily soluble at 1% in deionised. Alternatively, it can be reconstituted with a neutral buffer, such as PBS. |
SDS-PAGE : |
The preparation shows a tight cluster of bands with a molecular weight of 77 kDa. Dimers may appear under certan conditions. |
Protein : |
98% (By Lowry) |
Purity : |
> 98 % (By Cellulose Acetate Electrophoresis) |
Iron : |
27.85 µg/g (By Atomic Absorption). 1 gm transferrin can bind approximately 1400-1500 µg of iron. |
pH : |
7.24 (3% solution) |
Endotoxin Level : |
0.034 EU/mg (By Limulus Amebocyte Lysate) |
Microbial detection : |
No Mycoplasma is detected and Bioburden is less than 10 cfu/ml. |
Moisture : |
0.9 % (By Loss on Drying) |
Storage : |
Store at -10°C or below. |
Pathways : |
EPHB forward signaling; Formation of Platelet plug; HIF-1-alpha transcription factor network; Hemostasis; Iron uptake and transport; Mineral absorption; Platelet Activation; Platelet degranulation; Response to elevated platelet cytosolic Ca2+; Transferrin endocytosis and recycling; Transmembrane transport of small molecules |
PDBrendering based on 1a8e : |
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