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| Cat. No.: | TF-4777H |
| Product Overview: | Product is the lyophilized powder of human TF and buffer salts. Human TF is purified from pooled human serum using multi-step procedures which may include salt fractionation, gel filtration, ion-exchange chromatography and immunoabsorption. The product is dialyzed into 0.01M sodium phosphate, 0.07M sodium chloride, pH 7.3, filtered through a 0.22 µm filter, vialed and lyophilized. No preservative has been added. |
| Description: | TF is a glycoprotein that binds iron very tightly but reversibly. Although iron bound to transferrin is less than 0.1% (4 mg) of the total body iron, it is the most important iron pool, with the highest rate of turnover (25 mg/24 h). TF has a molecular weight of around 80 kDa and contains 2 specific high-affinity Fe (III) binding sites. |
| Source: | Human Plasma |
| Species: | Human |
| Preparation: | The protein is purified from pooled human plasma and lyophilized from 0.02M NH4HCO3. |
| Reconstitution: | The protein is readily soluble at 1% in deionised. Alternatively, it can be reconstituted with a neutral buffer, such as PBS. |
| SDS-PAGE: | The preparation shows a tight cluster of bands with a molecular weight of 77 kDa. Dimers may appear under certan conditions. |
| Protein: | 98% (By Lowry) |
| Purity: | > 98 % (By Cellulose Acetate Electrophoresis) |
| Iron: | 27.85 µg/g (By Atomic Absorption). 1 gm transferrin can bind approximately 1400-1500 µg of iron. |
| pH: | 7.24 (3% solution) |
| Endotoxin Level: | 0.034 EU/mg (By Limulus Amebocyte Lysate) |
| Microbial detection: | No Mycoplasma is detected and Bioburden is less than 10 cfu/ml. |
| Moisture: | 0.9 % (By Loss on Drying) |
| Storage: | Store at -10°C or below. |
| Pathways: | EPHB forward signaling; Formation of Platelet plug; HIF-1-alpha transcription factor network; Hemostasis; Iron uptake and transport; Mineral absorption; Platelet Activation; Platelet degranulation; Response to elevated platelet cytosolic Ca2+; Transferrin endocytosis and recycling; Transmembrane transport of small molecules |
| Gene Name: | TF transferrin [ Homo sapiens ] |
| Official Symbol: | TF |
| Synonyms: | TF; transferring; PRO1557; PRO2086; DKFZp781D0156; serotransferrin; siderophilin; OTTHUMP00000197155; beta-1 metal-binding globulin; Serotransferrin; Beta-1 metal-binding globulin; Siderophilin |
| Gene ID: | 7018 |
| mRNA Refseq: | NM_001063 |
| Protein Refseq: | NP_001054 |
| MIM: | 190000 |
| UniProt ID: | P02787 |
| Chromosome Location: | 3q21 |
| Function: | ferric iron binding; metal ion binding; protein binding; ubiquitin protein ligase binding |
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