||Recombinant Human IL-1RN produced inE. coliis a non-glycosylated, N-terminal methionyl form of the human naturally-occurring polypeptide chain containing 153 amino acids and having a molecular mass of 17258 Dalton.
||Interleukin-1 receptor antagonist (IL-1RN) is a member of the IL-1 family. Endogenous IL-1RN is produced in numerous animal disease models as well as in human autoimmune and chronic inflammatory diseases. It binds to IL-1 receptors in competition with IL-1, but does not elicit intracellular response from this binding. Its role in counteracting the proinflammatory effects of IL-1 is being studied by numerous research groups.
||>95% as determined by RP-HPLC, FPLC and SDS-PAGE.
||IL-1RN was lyophilized after extensive dialysis against PBS.
||The ED50 as determined by the dose-dependant inhibition of IL-1 stimulation of D10S cells was found to be 0.5 ng/ml, corresponding to a Specific Activity of 2.0 x 106IU/mg.
||Less than 0.1ng/µg (1IEU/µg) of IL-1RN.
||It is recommended to reconstitute the lyophilized rHuIL-1RN in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
||Lyophilized rHuIL-1RN although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution rHuIL-1RN should be stored at 4°C between 2-7 days and for future use below -18°C. For long-term storage it is recommended to add a carrier protein (0.1% HAS or BSA). Aliquot to avoid repeated freeze-thaw cycles.