||Recombinant Human IL-6 produced inE. coliis a single, non-glycosylated polypeptide chain containing 185 amino acids and having a molecular mass of 21.0 kDa.
||Interleukin (IL)-6 is an important proinflammatory and immunoregulatory cytokine expressed by various cells. Interleukin-6 has been shown to inhibit the growth of early stage and to promote the proliferation of advanced stage melanoma cells in vitro.
||>98% by SDS-PAGE, FPLC and RP-HPLC.
||Lyophilized from a concentrated (1mg/ml) solution in water containing 43µg/ml sodium chloride.
||rHuIL-6 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of human TF-1 cells is less than 0.1 ng/ml, corresponding to a Specific Activity of 5.0 x 107IU/mg.
||Less than 0.3ng/µg (0.3IEU/µg) determined by LAL test.
||It is recommended to reconstitute the lyophilized rHuIL-6 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
||Lyophilized rHuIL-6 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution rHuIL-6 should be stored at 4°C between 2-7 days and for future use below -18°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Aliquot to avoid repeated freeze-thaw cycles.