Recombinant Human Interleukin-6

Cat. No.: IL6-12H
Product Overview: Recombinant Human IL-6 produced inE. coliis a single, non-glycosylated polypeptide chain containing 185 amino acids and having a molecular mass of 21.0 kDa.
Description: Interleukin (IL)-6 is an important proinflammatory and immunoregulatory cytokine expressed by various cells. Interleukin-6 has been shown to inhibit the growth of early stage and to promote the proliferation of advanced stage melanoma cells in vitro.
Purity: >98% by SDS-PAGE, FPLC and RP-HPLC.
Formulation: Lyophilized from a concentrated (1mg/ml) solution in water containing 43µg/ml sodium chloride.
Specific Activity: rHuIL-6 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of human TF-1 cells is less than 0.1 ng/ml, corresponding to a Specific Activity of 5.0 x 107IU/mg.
Endotoxin: Less than 0.3ng/µg (0.3IEU/µg) determined by LAL test.
Reconstitution: It is recommended to reconstitute the lyophilized rHuIL-6 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Storage: Lyophilized rHuIL-6 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution rHuIL-6 should be stored at 4°C between 2-7 days and for future use below -18°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Aliquot to avoid repeated freeze-thaw cycles.
Gene Name: IL6
Synonyms: HGF; BSF2; HSF; IFNB2; IL-6; CDF; BSF-2; B-cell stimulatory factor 2; CTL differentiation factor; Hybridoma growth factor; Interferon beta-2; Interleukin-6 precursor; interleukin 6 (interferon, beta 2); IL6
Gene ID: 3569
mRNA Refseq: NM_000600.2
Protein Refseq: NP_000591.1
MIM: 147620
UniProt ID: P05231
Chromosome Location: 7p21
Pathway: Cytokine-cytokine receptor interaction, Hematopoietic cell lineage, Jak-STAT signaling pathway, Prion disease, Toll-like receptor signaling pathway
Function: Cytokine activity, Interleukin-6 receptor binding, Protein binding

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