||Recombinant Mouse IL-10 produced inE. coliis a single, non-glycosylated polypeptide chain containing 161 amino acids and having a molecular mass of 18885 Dalton.
||Interleukin-10 is produced by murine T-cells following their stimulation by lectins. The main source for B-cell-derived Interleukin-10 in mice is Ly-1 B-cells that express CD5 and CD11. Murine keratinocytes also produce IL10.
||>98% as determined by RP-HPLC, FPLC and SDS-PAGE
||The protein was lyophilized with no additives.
||The ED50 as determined by the dose-dependant co-stimulation with murine IL-4 of MC-9 cells was found to be < 2ng/ml, corresponding to a Specific Activity of 5.0 x 105IU/mg.
||Less than 0.1 ng/µg (IEU/µg) of mouse IL-10.
||It is recommended to reconstitute the lyophilized mouse IL-10 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
||Lyophilized mouse IL-10 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Murine IL-10 should be stored at 4°C between 2-7 days and for future use below -18°C. For long-term storage it is recommended to add a carrier protein (0.1% HAS or BSA). Aliquot to avoid repeated freeze-thaw cycles.