Recombinant Human Ubiquitin Conjugating Enzyme 12, His6-Tagged
Cat.No. : | UBE2M-05H |
Product Overview : | Recombinant Human Ubiquitin Conjugating Enzyme 12 produced inE.coliis a single non-glycosylated polypeptide chain containing 216 amino acids and having a molecular mass of approximately 25 kDa. |
- Specification
- Gene Information
- Related Products
- Download
Cat. No. : | UBE2M-05H |
Description : | UbcH12 is functional inin vitroNEDDylation reactions. It has been shown to form a thioester linkage with NEDD8 in the presence of the NEDD8 activating enzyme complex Uba3/APP-BP1. APP-BP1 binds to the amyloid precursor protein (APP) carboxy terminal domain and is important in conjunction with Uba3 and UbcH12 in driving cells through the S to M checkpoint. It was demonstrated to be the E2 responsible for the NEDDylation of the Cul-1 component of the SCF(β-TRCP) complex which is important as the E3-ligase in the ubiquitinylation of IκBα. NEDDylation of Cul-1 is essential for conjugation and processing of NF-κB p105 by SCF(β-TRCP) following phosphorylation of the complex. A dominant negative form of UbcH12, previously demonstrated to sequester NEDD8 and inhibit its conjugation, inhibits both conjugation and processing of p105, which is alleviated by wild-type UbcH12. |
Purity : | >95% by SDS-PAGE and HPLC analyses. |
Formulation : | Lyophilized from a 0.2μm filtered concentrated (1mg/ml) solution in 1×PBS, 1mM DTT, pH 7.5. |
Endotoxin : | Less than 1EU/μg of rHuUbcH12 as determined by LAL method. |
Reconstitution : | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20oC. Further dilutions should be made in appropriate buffered solutions. |
Storage : | This lyophilized preparation is stable at 2-8oC, but should be kept at -20oC for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8oC. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20oC to -70oC. Avoid repeated freeze/thaw cycles. |
Gene Name : | UBE2M |
Synonyms : | UBC12; hUbc12; UBC-RS2 ubiquitin-conjugating enzyme E |
Gene ID : | 9040 |
mRNA Refseq : | NM_003969 |
Protein Refseq : | NP_003960 |
MIM : | 603173 |
UniProt ID : | P61081 |
Chromosome Location : | 19q13.43 |
Function : | ligase activity;protein binding;ribosomal S6-glutamic acid ligase activity; ubiquitin-protein ligase activity; ubiquitin-protein ligase activity. |
Products Types
◆ Recombinant Protein | ||
UBE2M-4878R | Recombinant Rhesus Macaque UBE2M Protein, His (Fc)-Avi-tagged | +Inquiry |
UBE2M-857H | Recombinant Human UBE2M protein(Met1-Lys183) | +Inquiry |
UBE2M-001H | Recombinant Human UBE2M protein | +Inquiry |
UBE2M-6685H | Recombinant Human UBE2M Protein (Met1-Lys183), His tagged | +Inquiry |
UBE2M-5065R | Recombinant Rhesus monkey UBE2M Protein, His-tagged | +Inquiry |
◆ Lysates | ||
UBE2M-567HCL | Recombinant Human UBE2M 293 Cell Lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Inquiry
- Q&As
- Reviews
Q&As (0)
Ask a questionAsk a Question for All UBE2M Products
Required fields are marked with *
My Review for All UBE2M Products
Required fields are marked with *
0
Inquiry Basket