Recombinant Human Soluble CD4 Molecule

Cat. No.: CD4-99H
Product Overview: Recombinant Human sCD4 produced inBaculovirusis a single, glycosylated polypeptide chain containing 365 amino acids and having a non glycosilated molecular mass of 45 kDa. As a result of glycosilation the sCD4 migrates as a 46 kDa protein on standard SDS-PAGE. Detected By Western Immune Blots anti CD4 monoclonal antibody. The sCD4 is purified by proprietary chromatographic techniques.
Description: CD4 is a cell-surface glycoprotein found on the mature helper T cells and immature thymocytes, as well as on monocytes and macrophages. (Some cytotoxic T cells have CD4 protein as well.) Normally, about 65% of T cells in the blood are CD4+ (have CD4 protein protruding from their membrane). A mature T cells with either have CD4 or CD8, but not both. During one stage of development T cells develop CD4 and CD8 receptors, but they eventually are differentiated in the thymus and become more specialized.
Source: Baculovirus
Amino Acid Sequence: The sequence of the amino acids was determined and was found to be: KKVVL GKKGDTVELT CTASQKKSIQ FHWKNSNQIK ILGNQGSFLT KGPSKLNDRA DSRRSLWDQG NFPLIIKNLK IEDSDTYICE VEDQKEEVQL LVFGLTANSD THLLQGQSLT LTLESPPGSS PSVQCRSPRG KNIQGGKTLS VSQLELQDSG TWTCTVLQNQ KKVEFKIDIV VLAFQKASSI VYKKEGEQVE FSFPLAFTVE KLTGSGELWWQAERASSSKS WITFDLKNKE VSVKRVTQDP KLQMGKKLPL HLTLPQALPQ YAGSGNLTLALEAKTGKLHQ EVNLVVMRAT QLQKNLTCEV WGPTSPKLML SLKLENKEAK VSKREKAVWV LNPEAGMWQC LLSDSGQVLL ESNIKVLPTW
Physical Appearance: Sterile Filtered colorless solution (259μg/ml).
Purity: Greater than 90.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Formulation: The solution contains 10mM sodium phosphate buffer pH-7.0, 300 mM NaCl and 0.01% tween-80.
Biological Activity: Immune reactive in ELISA using monoclonal anti CD4 in gpl2O capture ELISA assays.
Storage: sCD4 Human Recombinant although stable at 14°C for 1 week, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Gene Name: CD4 CD4 molecule [ Homo sapiens ]
Synonyms: CD4; CD4 molecule; CD4mut; T-cell surface glycoprotein CD4; CD4 antigen (p55); T-cell surface antigen T4/Leu-3; CD antigen; CD4 antigen; CD4 receptor
Gene ID: 920
mRNA Refseq: NM_000616
Protein Refseq: NP_000607
MIM: 186940
UniProt ID: P01730
Chromosome Location: 12pter-p12
Pathway: Antigen processing and presentation; Cell adhesion molecules (CAMs); Hematopoietic cell lineage; Primary immunodeficiency; T cell receptor signaling pathway; HIV Infection; Signaling in Immune system
Function: MHC class II protein binding; coreceptor activity; extracellular matrix structural constituent; protein homodimerization activity; protein kinase binding; transmembrane receptor activity; zinc ion binding

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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