||Recombinant Human sCD4 produced inBaculovirusis a single, glycosylated polypeptide chain containing 365 amino acids and having a non glycosilated molecular mass of 45 kDa. As a result of glycosilation the sCD4 migrates as a 46 kDa protein on standard SDS-PAGE. Detected By Western Immune Blots anti CD4 monoclonal antibody. The sCD4 is purified by proprietary chromatographic techniques.
||CD4 is a cell-surface glycoprotein found on the mature helper T cells and immature thymocytes, as well as on monocytes and macrophages. (Some cytotoxic T cells have CD4 protein as well.) Normally, about 65% of T cells in the blood are CD4+ (have CD4 protein protruding from their membrane). A mature T cells with either have CD4 or CD8, but not both. During one stage of development T cells develop CD4 and CD8 receptors, but they eventually are differentiated in the thymus and become more specialized.
|Amino Acid Sequence:
||The sequence of the amino acids was determined and was found to be: KKVVL GKKGDTVELT CTASQKKSIQ FHWKNSNQIK ILGNQGSFLT KGPSKLNDRA DSRRSLWDQG NFPLIIKNLK IEDSDTYICE VEDQKEEVQL LVFGLTANSD THLLQGQSLT LTLESPPGSS PSVQCRSPRG KNIQGGKTLS VSQLELQDSG TWTCTVLQNQ KKVEFKIDIV VLAFQKASSI VYKKEGEQVE FSFPLAFTVE KLTGSGELWWQAERASSSKS WITFDLKNKE VSVKRVTQDP KLQMGKKLPL HLTLPQALPQ YAGSGNLTLALEAKTGKLHQ EVNLVVMRAT QLQKNLTCEV WGPTSPKLML SLKLENKEAK VSKREKAVWV LNPEAGMWQC LLSDSGQVLL ESNIKVLPTW
||Sterile Filtered colorless solution (259μg/ml).
||Greater than 90.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
||The solution contains 10mM sodium phosphate buffer pH-7.0, 300 mM NaCl and 0.01% tween-80.
||Immune reactive in ELISA using monoclonal anti CD4 in gpl2O capture ELISA assays.
||sCD4 Human Recombinant although stable at 14°C for 1 week, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.