Recombinant Human SAE1 protein(Met1-Lys346), His&GST-tagged

• Cat.No.: SAE1-8623H
• Product Overview: Recombinant Human SAE1 (Q9UBE0) (Met1-Lys346) was expressed in Insect Cells, fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Specification

• Source: Insect Cells
• Species: Human
• Tag: N-His&GST
• Protein length: Met1-Lys346
• Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% gly Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant human SAE1 /GST chimera consists of 583 amino acids and has a calculated molecular mass of 66.3 kDa. The recombinant protein migRates as an approximately 61 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: SAE1 SUMO1 activating enzyme subunit 1 [ Homo sapiens ]
• Official Symbol: SAE1
• Synonyms: SAE1; SUMO1 activating enzyme subunit 1; SUMO-activating enzyme subunit 1; activator Of sumo 1; AOS1; FLJ3091; Sua1; activator of SUMO1; SUMO-1 activating enzyme subunit 1; sentrin/SUMO-activating protein AOS1; SUMO-1 activating enzyme E1 N subunit; ubiquitin-like 1-activating enzyme E1A; ubiquitin-like protein SUMO-1 activating enzyme; SUA1; UBLE1A; HSPC140
• Gene ID: 10055
• mRNA Refseq: NM_001145713
• Protein Refseq: NP_001139185
• MIM: 613294
• UniProt ID: Q9UBE0