Recombinant Mouse F7, His-tagged

• Cat.No.: F7-8662M
• Product Overview: A DNA sequence encoding the mouse FⅦ (NP_034302.2) (Met 1-Leu 446) was fused with the a polyhistidine tag at the C-terminus.

Specification

• Description: Coagulation factor VII, also known as Serum prothrombin conversion accelerator, Factor VII, F7 and FVII, is a member of the peptidase S1 family. Factor VII is one of the central proteins in the coagulation cascade. It is an enzyme of the serine protease class. Factor VII contains two EGF-like domains, one Gla (gamma-carboxy-glutamate) domain and one peptidase S1 domain. The main role of factor VII is to initiate the process of coagulation in conjunction with tissue factor (TF). Tissue factor is found on the outside of blood vessels, normally not exposed to the blood stream. The action of the Factor VII is impeded by tissue factor pathway inhibitor (TFPI), which is released almost immediately after initiation of coagulation. Factor VII is vitamin K dependent and is produced in the liver. The use of warfarin or similar anticoagulants impairs its function. Upon vessel injury, tissue factor is exposed to the blood and circulating Factor VII. Once bound to TF, FVII is activated to FVIIa by different proteases, among which are thrombin (factor IIa), factor Xa, IXa, XIIa, and the FVIIa-TF complex itself. The most important substrates for FVIIa-TF are Factor X and Factor IX.
• Source: Human Cells
• Species: Mouse
• Tag: His
• Predicted N Terminal: Val 25 & Ala 42
• Form: Lyophilized from sterile PBS, pH7.4. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization.
• Molecular Mass: The mature form of mouse FⅦ consists of 416 amino acids after removal of the signal peptide and the propeptid, and has a predicted molecular mass of 47 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm FⅦ is approximately 56-63 kDa due to glycosylation.
• Endotoxin: 1.0 eu/μg of the protein as determined by the lal
• Purity: >90 % as determined by SDS-PAGE
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: F7 coagulation factor VII [ Mus musculus ]
• Official Symbol: F7
• Synonyms: F7; coagulation factor VII; serum prothrombin conversion accelerator; Cf7; FVII; mfVII; AI132620
• Gene ID: 14068
• mRNA Refseq: NM_010172
• Protein Refseq: NP_034302
• Pathway: Blood Clotting Cascade, organism-specific biosystem; Complement and Coagulation Cascades, organism-specific biosystem; Complement and coagulation cascades, organism-specific biosystem; Complement and coagulation cascades, conserved biosystem; Extrinsic Pathway, organism-specific biosystem; Formation of Fibrin Clot (Clotting Cascade), organism-specific biosystem; Gamma-carboxylation of protein precursors, organism-specific biosystem
• Function: calcium ion binding; catalytic activity; endopeptidase activity; glycoprotein binding; hydrolase activity; peptidase activity; receptor binding; serine-type endopeptidase activity; serine-type peptidase activity