| Description : |
Glial cell line derived neurotrophic factor (GDNF) is a protein promoting the survival of various neuronal subpopulations at different stages of development. These include dopaminergic neurons in midbrain, GDNF stimulates their survival, differentiation and high-affinity dopamine uptake. The use of GDNF in the treatment of Parkinson''''s disease and spinal cord injuries has shown promising results. GDNF belongs to the TGF-ß superfamily, it is a homodimeric protein with the familial cysteine-knot structure. |
| Source : |
Hordeum vulgare |
| Species : |
Human |
| Predicted N Terminal : |
His |
| Form : |
Sterile filtered through a 0.2 µm filter. Lyophilized from PBS, pH 7.2 |
| Bio-activity : |
The bioassay measures the ability of GDNF to differentiate mouse Embryonic Stem Cells into dopaminergic neurons (TH positive). Once fixed, the cells are immunostained for Tuj1(a marker for common neurons), TH (marker for dopaminergic neurons) and the nuclear dye Dapi and the TH positive cells in each Tuj1 postive colony are estimated (Barberi et al, 2003).rh GDNF at concentration of 20 ng/ml succesfully differentiated mouse ES cells to dopaminergic neurons and outperformed GDNF from another commercial source. Lowest limit of active GDNF concentration was not determined. |
| Molecular Mass : |
Recombinant human GDNF contains 134 amino acids and a 16 a.a. histidine-based tag for a total length of 150 a.a. and has a predicted molecular mass of 17.2 kDa including his-tag. As a result of glycosylation, the recombinant protein migrates with an apparent molecular mass of 25 kDa in SDS-PAGE. |
| Endotoxin : |
Endotoxin level is less than 0.05EU/µg as measured by turbidimetric kinetic assay. |
| Purity : |
> 95% by SDS-PAGE gel analysis. |
| Storage : |
The lyophilized protein, though stable at room temperature for few weeks, is best stored at -20°C. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles. |
| Reconstitution : |
It is recommended to reconstitute the lyophilized protein in sterile water to a concentration of no less than 100 µg/ml. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). |
