Recombinant Human ADCY6 protein, GST-tagged
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|Product Overview :||Recombinant Human ADCY6 (310-655aa) protein was fused to GST-tag and expressed in E.coli.|
|Description :||This gene encodes a member of the adenylyl cyclase family of proteins, which are required for the synthesis of cyclic AMP. All members of this family have an intracellular N-terminus, a tandem repeat of six transmembrane domains separated by a cytoplasmic loop, and a C-terminal cytoplasmic domain. The two cytoplasmic regions bind ATP and form the catalytic core of the protein. Adenylyl cyclases are important effectors of transmembrane signaling pathways and are regulated by the activity of G protein coupled receptor signaling. This protein belongs to a small subclass of adenylyl cyclase proteins that are functionally related and are inhibited by protein kinase A, calcium ions and nitric oxide. A mutation in this gene is associated with arthrogryposis multiplex congenita.|
|Form :||Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH8.0). 5% trehalose and 5% mannitol are added as protectants before lyophilization.|
|Molecular Mass :||64 kDa|
|AA Sequence :||AEVSQRQAFQ ETRGYIQARL HLQHENRQQE RLLLSVLPQH VAMEMKEDIN TKKEDMMFHK IYIQKHDNVS ILFADIEGFT SLASQCTAQE LVMTLNELFA RFDKLAAENH CLRIKILGDC YYCVSGLPEA RADHAHCCVE MGVDMIEAIS LVREVTGVNV NMRVGIHSGR VHCGVLGLRK WQFDVWSNDV TLANHMEAGG RAGRIHITRA TLQYLNGDYE VEPGRGGERN AYLKEQHIET FLILGASQKR KEEKAMLAKL QRTRANSMEG LMPRWVPDRA FSRTKDSKAF RQMGIDDSSK DNRGTQDALN PEDEVDEFLS RAIDARSIDQ LRKDHVRRFL LTFQRE|
|Purity :||85%, by SDS-PAGE with Coomassie Brilliant Blue staining.|
|Storage :||Short-term storage: Store at 2-8 centigrade for (1-2 weeks).
Long-term storage: Aliquot and store at -20 to -80 centigrade for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
|Reconstitution :||Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage.
If a different concentration is needed for your purposes please adjust the reconstitution volume as required (please note: the ion concentration of the final solution will vary according to the volume used).
Note: Centrifuge vial before opening. When reconstituting, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution.
|Shipping :||The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.|
|SDS-PAGE of ADCY6-9409H :||
|Gene Name :||ADCY6|
|Official Symbol :||ADCY6|
|Synonyms :||AC6; LCCS8|
|Gene ID :||112|
|mRNA Refseq :||NM_015270.5|
|Protein Refseq :||NP_056085.1|
|UniProt ID :||O43306|
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