Recombinant Human CRK, GST-tagged

• Cat.No.: CRK-11582H
• Product Overview: Recombinant Human CRK protein, fused to GST-tag, was expressed in E.coli and purified by GSH-sepharose.

Specification

• Description: This gene encodes a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. The product of this gene has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Two alternative transcripts encoding different isoforms with distinct biological activity have been described.
• Source: E.coli
• Species: Human
• Tag: GST
• Protein length: C-term-264a.a.
• Storage: The protein is stored in PBS buffer at -20℃. Avoid repeated freezing and thawing cycles.
• Storage Buffer: 1M PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH8. ) added with 100mM GSH and 1% Triton X-100,15%glycerol.

Gene Information

• Gene Name: CRK v-crk sarcoma virus CT10 oncogene homolog (avian) [ Homo sapiens ]
• Official Symbol: CRK
• Synonyms: CRK; v-crk sarcoma virus CT10 oncogene homolog (avian); v crk avian sarcoma virus CT10 oncogene homolog; adapter molecule crk; proto-oncogene c-Crk; p38; CRKII; FLJ38130
• Gene ID: 1398
• mRNA Refseq: NM_005206
• Protein Refseq: NP_005197
• MIM: 164762
• UniProt ID: P46108
• Chromosome Location: 17p13
• Pathway: ARMS-mediated activation, organism-specific biosystem; Angiopoietin receptor Tie2-mediated signaling, organism-specific biosystem; B Cell Receptor Signaling Pathway, organism-specific biosystem; Bacterial invasion of epithelial cells, organism-specific biosystem; Bacterial invasion of epithelial cells, conserved biosystem; CXCR4-mediated signaling events, organism-specific biosystem; Chemokine signaling pathway, organism-specific biosystem
• Function: SH2 domain binding; SH3/SH2 adaptor activity; ephrin receptor binding; protein binding; protein phosphorylated amino acid binding

Reviews

04/17/2022

The expression is high and the detection is convenient.

08/22/2020

The purity and biological activity are excellent, which makes the products extremely reliable.

07/13/2020

The background in the Western blot is very clean and shows good antibody specificity.

Q&As

How is the role of CRK assessed in the disease?

The role of CRK in disease can be assessed through in-depth analysis of the patient's genomic data, combined with the results of studies in cell models and animal models. This can help doctors better understand the patient's condition and develop more effective treatment plans.

How can I detect the expression level of CRK?

The expression level of CRK can be detected by immunohistochemistry, western blotting, and real-time PCR. These techniques can detect protein or mRNA levels of CRK in tissues or cells to assess changes in its expression in disease.

Which biomolecules can be used as molecular markers for CRK?

Certain biomolecules can be used as molecular markers for CRK, such as mRNA or protein of CRK detected in biological samples such as blood and urine. These markers can be used for early diagnosis of disease, prognostic judgment, or monitoring of treatment efficacy.

What are the detection methods for CRK?

Detection methods for CRK include immunohistochemistry, western blotting, real-time PCR, etc. These methods can detect the expression level of CRK in tissues or cells to assess its status in disease.

What are the treatment strategies for CRK?

Therapeutic strategies for CRK include inhibition of its activity, regulation of its expression, and gene therapy. For example, the development of small molecule inhibitors or antibody drugs against CRK to inhibit its aberrant activity in disease, or the regulation of CRK expression through gene knockout or overexpression techniques.

What is the main role of CRK in cells?

As a signal transduction molecule, CRK plays multiple roles within the cell. It can phosphorylate a variety of substrates, affecting their activity or localization. In addition, CRK is also involved in processes such as cytoskeletal reorganization and cell motility.