Recombinant Human CRK 293 Cell Lysate

• Cat.No.: CRK-7277HCL

Specification

• Description: Antigen standard for v-crk sarcoma virus CT10 oncogene homolog (avian) (CRK), transcript variant I is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
• Tag: Non

Gene Information

• Gene Name: CRK v-crk sarcoma virus CT10 oncogene homolog (avian) [ Homo sapiens ]
• Official Symbol: CRK
• Synonyms: CRK; v-crk sarcoma virus CT10 oncogene homolog (avian); v crk avian sarcoma virus CT10 oncogene homolog; adapter molecule crk; proto-oncogene c-Crk; p38; CRKII; FLJ38130
• Gene ID: 1398
• mRNA Refseq: NM_005206
• Protein Refseq: NP_005197
• MIM: 164762
• UniProt ID: P46108
• Chromosome Location: 17p13
• Pathway: ARMS-mediated activation, organism-specific biosystem; Angiopoietin receptor Tie2-mediated signaling, organism-specific biosystem; B Cell Receptor Signaling Pathway, organism-specific biosystem; Bacterial invasion of epithelial cells, organism-specific biosystem; Bacterial invasion of epithelial cells, conserved biosystem; CXCR4-mediated signaling events, organism-specific biosystem; Chemokine signaling pathway, organism-specific biosystem
• Function: SH2 domain binding; SH3/SH2 adaptor activity; ephrin receptor binding; protein binding; protein phosphorylated amino acid binding

Reviews

04/17/2022

The expression is high and the detection is convenient.

08/22/2020

The purity and biological activity are excellent, which makes the products extremely reliable.

07/13/2020

The background in the Western blot is very clean and shows good antibody specificity.

Q&As

How is the role of CRK assessed in the disease?

The role of CRK in disease can be assessed through in-depth analysis of the patient's genomic data, combined with the results of studies in cell models and animal models. This can help doctors better understand the patient's condition and develop more effective treatment plans.

How can I detect the expression level of CRK?

The expression level of CRK can be detected by immunohistochemistry, western blotting, and real-time PCR. These techniques can detect protein or mRNA levels of CRK in tissues or cells to assess changes in its expression in disease.

Which biomolecules can be used as molecular markers for CRK?

Certain biomolecules can be used as molecular markers for CRK, such as mRNA or protein of CRK detected in biological samples such as blood and urine. These markers can be used for early diagnosis of disease, prognostic judgment, or monitoring of treatment efficacy.

What are the detection methods for CRK?

Detection methods for CRK include immunohistochemistry, western blotting, real-time PCR, etc. These methods can detect the expression level of CRK in tissues or cells to assess its status in disease.

What are the treatment strategies for CRK?

Therapeutic strategies for CRK include inhibition of its activity, regulation of its expression, and gene therapy. For example, the development of small molecule inhibitors or antibody drugs against CRK to inhibit its aberrant activity in disease, or the regulation of CRK expression through gene knockout or overexpression techniques.

What is the main role of CRK in cells?

As a signal transduction molecule, CRK plays multiple roles within the cell. It can phosphorylate a variety of substrates, affecting their activity or localization. In addition, CRK is also involved in processes such as cytoskeletal reorganization and cell motility.