Recombinant Rat Il33 protein
Cat.No. : | Il33-583R |
Product Overview : | Recombinant Rat Il33 protein was expressed in Escherichia coli. |
- Specification
- Gene Information
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Description : | Interleukin-33 (IL-33), also known as NF-HEV and DVS 27, is a cytokine belonging to the IL-1 superfamily. It is also a proinflammatory protein that may regulate gene transcription and it induces helper T cells, mast cells, eosinophils and basophils to produce type 2 cytokines. The induction of type 2 cytokines by IL-33 in vivo is believed to induce the severe pathological changes observed in mucosal organs following administration of IL-33. IL-33 is constitutively expressed in smooth muscle and airway epithelia and it binds to a high-affinity receptor family member ST2. In vivo administration of mature IL-33 promotes increased production of IL-5, IL-13, IgE, and IgA, as well as splenomegaly and inflammatory infiltration of mucosal tissues. Recombinant rat IL-3 contains 156 amino acid residues and it shares 59 % a.a. and 90 % sequence identity with human and murine IL-33. |
Source : | E.coli |
Species : | Rat |
Form : | Lyophilized from a 0.2μm filtered concentrated solution in 20 mM Tris, 300 mM NaCl, pH 8.5. |
Bio-activity : | Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 × 10⁶ IU/mg. |
Molecular Mass : | Approximately 17.4 kDa, a single non-glycosylated polypeptide chain containing 156 amino acids. |
Protein length : | 156 |
AA Sequence : | SIQGTSLLTESCALSTYNDQSVSFV LENGCYVINVEDCGKNQEKDKVLLR YYESSFPAQSGDGVDGKKLMVNMSP IKDTDIWLNANDKDYSVELQKGDVS PPDQAFFVLHKKSSDFVSFECKNLP GTYIGVKDNQLALVEENDESCNNIM FKLSKM |
Endotoxin : | Less than 1 EU/µg of rRtIL-33 as determined by LAL method. |
Purity : | >95% by SDS-PAGE and HPLC analysis. |
Storage : | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution. |
Reconstitution : | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions. |
Gene Name : | Il33 |
Official Symbol : | Il33 |
Synonyms : | IL-33 |
Gene ID : | 361749 |
mRNA Refseq : | NM_001014166 |
Protein Refseq : | NP_001014188 |
UniProt ID : | Q66H70 |
Products Types
◆ Recombinant Protein | ||
Il33-026I | Active Recombinant Mouse Il33 Protein (158 aa) | +Inquiry |
Il33-192M | Recombinant Active Mouse IL33 Protein, His-tagged(C-ter) | +Inquiry |
Il33-3521M | Recombinant Mouse Il33 Protein | +Inquiry |
IL33-1917M | Recombinant Mouse IL33 Protein | +Inquiry |
IL33-356I | Active Recombinant Human IL33 Protein (159 aa) | +Inquiry |
◆ Lysates | ||
IL33-5226HCL | Recombinant Human IL33 293 Cell Lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Inquiry
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Q&As (6)
Ask a questionIt can be used to detect immune cells such as macrophages and dendritic cells.
This protein applied in immunology, drug research, life science and other fields.
Regulates the activity of immune cells by binding to receptors to stimulate the production of inflammatory factors such as interferon and TNF-α.
IL33 can also be used to treat a variety of inflammatory diseases, such as inflammatory bowel disease, psoriasis and so on.
The preparation of IL33 requires first cloning the IL33 gene into the expression vector, and then the expression vector is expressed and purified in the cell culture medium.
Treating asthma with recombinant IL33 takes several weeks to several months to be effective, depending on the severity of the condition and the individual patient.
Customer Reviews (3)
Write a reviewGood solubility and is easy to operate and purify.
Effectively mimics the function of the target protein in vitro.
IL33 has a wide range of applications in the field of scientific research and is suitable for a variety of experimental design and research purposes.
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