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Recombinant Rat Ccl28

Cat.No.: Ccl28-639R
Product Overview: Recombinant Rat Ccl28 produced in E. coli is approximately 13.1 kDa, a single non-glycosylated polypeptide chain containing 116 amino acids.
Description: Mucosae-associated Epithelial Chemokine (MEC)/CCL28 (CC chemokine ligand 28) is a secreted CC chemokine expressed primarily by epithelial cells of the bronchioles, salivary gland, mammary gland and colon. MEC signals through the CCR10 receptor and chemoattracts resting CD4, CD8 T-cells and eosinophils. MEC contains six cysteines including the four highly conserved cysteine residues present in CC chemokines.
Source: E. coli
Species: Rat
Form: Lyophilized from a 0.2 μm filtered concentrated solution in 20mM PB, pH 7.4, 200mM NaCl.
Bio-activity: Fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human lymphocytes is in a concentration range of 5.0-50 ng/ml.
Molecular Mass: 13.1 kDa
AA Sequence: SEAILPIASS CCTEVSHHIP RRLLERVNSC SIQRADGDCD LAAVILHVKR RRICVSPHNP TLKRWMSASE MKNGKENLCP RKKQDSGKDR KGHTPRKHGK HGTRRIHGTH DHEAPR
Endotoxin: Less than 1 EU/μg of rRtMEC/CCL28 as determined by LAL method.
Purity: >96% by SDS-PAGE and HPLC analyses.
Usage: This material is for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
Storage: This lyophilized preparation is stable at 2-8 centigrade, but should be kept at -20 centigrade for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 centigrade. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 centigrade to -70 centigrade. Avoid repeated freeze/thaw cycles.
Reconstitution: We recommend that this vial be briefy centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 centigrade. Further dilutions should be made in appropriate buffered solutions.
Gene Name: Ccl28 chemokine (C-C motif) ligand 28 [ Rattus norvegicus ]
Official Symbol: Ccl28
Synonyms: CCL28; chemokine (C-C motif) ligand 28; C-C motif chemokine 28; CC chemokine CCL28; Scya28;
Gene ID: 114492
mRNA Refseq: NM_053700
Protein Refseq: NP_446152
Pathway: Chemokine receptors bind chemokines, organism-specific biosystem; Chemokine signaling pathway, organism-specific biosystem; Chemokine signaling pathway, conserved biosystem; Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; G alpha (i) signalling events, organism-specific biosystem;
Function: molecular_function;

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