Active Recombinant Human VEGFA

• Cat.No.: VEGFA-504H
• Product Overview: rHu Vascular Endothelial Growth Factor expressed in CHO cells is a double, glycosylated, polypeptide chain containing full lenght VEGF containing 165 amino acids (VEGF-165).

Specification

• Description: Recombinant Human Vascular Endothelial Growth Factor-A is expressed at high levels in certain tumor-derived cells but is produced by a wide variety of cell types. In contrast to other factors mitogenic for endothelial cells such as FGF-1, FGF-2 and PDGF, VEGF is synthesized as a precursor containing a typical hydrophobic secretory signal sequence of 26 amino acids. Glycosylation is not required for efficient secretion of VEGF. rHu VEGF-121 produced in E. coli is a double, non-glycosylated, polypeptide chain that contains 121 amino acids and has a molecular mass of 28.42 kDa.
• Source: CHO Cells (Chinese Hamster Ovary Cells)
• Species: Human
• Form: The protein was lyophilized from a concentrated (1mg/mL) solution with no additives.
• Bio-activity: Determined by its ability to stimulate 3H-Thymidine incorporation in human umbilical vein endothelial cells. The ED50 for this effect was found to be 2-6 ng/ml.
• Molecular Mass: 42,000 Da (by non-reducing SDS-PAGE migration)
• Purity: Purified by proprietary chromatographic techniques. SDS PAGE: Not more than 3% total contaminants. RP-HPLC: principal peak>97%.
• Stability: Lyophilized Vascular Endothelial Growth Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution, rh VEGF should be stored at 4oC between 2-7 days. For long term storage, it is recommended to add
• Reconstitution: It is recommended to reconstitute the lyophilized Vascular Endothelial Growth Factor Human in sterile nanopure water to not less than 100μg/ml. It can then be further diluted in other aqueous solutions.
• Warning: Please avoid freeze-thaw cycles

Gene Information

• Gene Name: VEGFA vascular endothelial growth factor A [ Homo sapiens ]
• Official Symbol: VEGFA
• Synonyms: VEGFA; vascular endothelial growth factor A; vascular endothelial growth factor , VEGF; VEGF A; VPF; vascular permeability factor; VEGF; MVCD1; MGC70609
• mRNA Refseq: NM_001025366
• Protein Refseq: NP_001020537
• UniProt ID: P15692
• Chromosome Location: 6p12
• Pathway: Bladder cancer, organism-specific biosystem; Bladder cancer, conserved biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Endochondral Ossification, organism-specifi
• Function: cell surface binding; chemoattractant activity; cytokine activity; cytokine activity; extracellular matrix binding; fibronectin binding; growth factor activity; growth factor activity; heparin binding; heparin binding; platelet-derived growth factor recep