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Recombinant Mouse Gpc3, His-tagged

Cat.No.: Gpc3-119M
Product Overview: A DNA sequence encoding the mouse GPC3 (Met1-Pro558) was expressed with a C-terminal polyhistidine tag.
Description: lypican-3 is a protein that in humans is encoded by the GPC3 gene. The protein encoded by this gene is a member of theglypican family.
Source: Baculovirus-Insect cells
Species: Mouse
Tag: His
Predicted N Terminal: Gln 25
Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 7.4.
Molecular Mass: The recombinant mouse GPC3 comprises 544 amino acids and has a predicted molecular mass of 62 kDa. The apparent molecular mass of the protein is approximately 33-37 kDa in SDS-PAGE under reducing conditions.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
Purity: > 95 % as determined by SDS-PAGE
Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Gene Name: Gpc3 glypican 3 [ Mus musculus (house mouse) ]
Official Symbol: Gpc3
Synonyms: Gpc3; OCI-5; glypican 3; glypican-3
Gene ID: 14734
mRNA Refseq: NM_016697
Protein Refseq: NP_057906
UniProt ID: Q8CFZ4
Chromosome Location: X; X A3.3
Pathway: A tetrasaccharide linker sequence is required for GAG synthesis; Chondroitin sulfate/dermatan sulfate metabolism; Glycosaminoglycan metabolism
Function: heparan sulfate proteoglycan binding; peptidase inhibitor activity; peptidyl-dipeptidase inhibitor activity

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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