Recombinant Human CD3E, His-tagged, Biotinylated

• Cat.No.: CD3E-275H
• Product Overview: Human CD3E /CD3 Epsilon Protein, Biotinylated (Biotinylated CD3E) Asp 23 - Asp 126, was labeled with biotin based on rhCD3E Protein, which was produced in human 293 cells (HEK293).?

Specification

• Description: The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women.
• Source: HEK293
• Species: Human
• Tag: His
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Trehalose are added as protectants before lyophilization.
• Molecular Mass: Biotinylated CD3E, fused with 6×His tag at the C-terminus, has a calculated MW of 13.2 kDa. The predicted N-terminus is Trp 22. DTT-reduced Protein migrates as 18-20 kDa due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg of the Biotinylated CD3E by the LAL method.
• Purity: >95% as determined by reduced SDS-PAGE.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).

Gene Information

• Gene Name: CD3E CD3e molecule, epsilon (CD3-TCR complex) [ Homo sapiens ]
• Official Symbol: CD3E
• Synonyms: CD3E; CD3e molecule, epsilon (CD3-TCR complex); CD3e antigen, epsilon polypeptide (TiT3 complex); T-cell surface glycoprotein CD3 epsilon chain; CD3-epsilon; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell antigen receptor complex, epsilon subunit of T3; T3E; TCRE; FLJ18683
• Gene ID: 916
• mRNA Refseq: NM_000733
• Protein Refseq: NP_000724
• UniProt ID: P07766
• Chromosome Location: 11q23
• Pathway: Adaptive Immune System, organism-specific biosystem; CXCR4-mediated signaling events, organism-specific biosystem; Chagas disease (American trypanosomiasis), organism-specific biosystem; Chagas disease (American trypanosomiasis), conserved biosystem; Costimulation by the CD28 family, organism-specific biosystem; Downstream TCR signaling, organism-specific biosystem; Downstream signaling in naive CD8+ T cells, organism-specific biosystem
• Function: SH3 domain binding; T cell receptor binding; protein heterodimerization activity; protein kinase binding; receptor activity; receptor signaling complex scaffold activity; receptor signaling protein activity; transmembrane signaling receptor activity