||Recombinant Mouse PCSK9 MS Standard Protein, C13 and N15-labeled (Mouse PCSK9, Heavy Labeled) Gln 35 - Gln 694 (Accession # AAH38085.1) was produced in human 293 cells (HEK293).
||Proprotein convertase subtilisin/kexin type 9 (PCSK9), also known as NARC1 (neural apoptosis regulated convertase), is a newly identified subtilase belonging to the peptidase S8 subfamily. Mouse PCSK9 is synthesized as a soluble zymogen, and undergoes autocatalytic intramolecular processing in the endoplasmic reticulum, resulting in the cleavage of its propeptide that remains associated with the secreted active enzyme with a broad alkaline pH optimum. This protein plays a major regulatory role in cholesterol homeostasis. PCSK9 binds to the epidermal growth factor-like repeat A (EGF-A) domain of the low-density lipoprotein receptor (LDLR), inducing LDLR degradation. PCSK9 may also have a role in the differentiation of cortical neurons. Mutations in this gene have been associated with a rare form of autosomal dominant familial hypercholesterolemia (HCHOLA3).[2-3]
||Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.
||Mouse PCSK9, Heavy Labeled, fused with 6×His tag at the C-terminus, has a calculated MW of 72 kDa. The predicted N-terminus is Gln 35. DTT-reduced Protein migrates as 20 kDa and 64 kDa due to glycosylation and proteolytic digestion.
||Less than 1.0 EU per μg of the Mouse PCSK9, Heavy Labeled by the LAL method.
||>97% as determined by SDS-PAGE.
||Avoid repeated freeze-thaw cycles.No activity loss was observed after storage at:In lyophilized state for 1 year (4oC-8oC); After reconstitution under sterile conditions for 1 month (4oC-8oC) or 3 months (-20oC to -70oC).