||Recombinant Human MCL1 was expressed as gst-tagged fusion protein by E.Coli and purified by GSH-sepharose.
||This gene encodes an anti-apoptotic protein, which is a member of the Bcl-2 family. Alternative splicing results in multiple transcript variants. The longest gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene products (isoform 2 and isoform 3) promote apoptosis and are death-inducing.
||The protein is stored in PBS buffer at -20 oC. Repeated freeze-thaw cycles should be avoided.
||1M PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH8. ) added with 100mM GSH and 1% Triton X-100,15%glycerol.