"USP8" Related Products


Recombinant Human USP8, FLAG-tagged

Cat.No. : USP8-86H
Product Overview : Human USP8, amino-acids 2-1118(end) with an N-terminal FLAG-tag, MW=128 kDa, expressed in a baculovirus-infected Sf9 cell expression system.
Description : Ubiquitin carboxyl-terminal hydrolase 8 is an enzyme that in humans is encoded by the USP8 gene.
Source : Sf9 cell
Species : Human
Tag : FLAG
Form : 45 mM Tris-HCl, pHUSP8 gel 8.0, 124 mM NaCl, 2.4 mM KCl, 90 μg/ml Flag peptide, 10% glycerol, and 3 mM DTT.
Bio-activity : 246 pmol/min/μg Assay conditions: Reaction was performed in 50 mM Tris, pH 7.4, 1 mM DTT, 0.5 mM EDTA, 500 nM Ub-AMC, and USP8 in a 50 μL reaction. Reaction was incubated at 37°C for 15 min and fluorescent signal was measured at excitation= 340 nm, emission = 460 nm.
Molecular Mass : 128 kDa
Purity : >70%
Applications : Useful for the study of enzymekinetics, screening inhibitors, and selectivityprofiling.
Stability : >6 months at –80°C
Gene Name : USP8 ubiquitin specific peptidase 8 [ Homo sapiens ]
Official Symbol : USP8
Synonyms : USP8; ubiquitin specific peptidase 8; ubiquitin specific protease 8; ubiquitin carboxyl-terminal hydrolase 8; HumORF8; KIAA0055; UBPY; Deubiquitinating enzyme 8; FLJ34456; hUBPY; HumORF8; KIAA0055; MGC129718; Ubiquitin carboxyl terminal hydrolase 8; Ubiquitin carboxyl-terminal hydrolase 8; Ubiquitin isopeptidase Y; ubiquitin specific peptidase 8; Ubiquitin specific processing protease 8; Ubiquitin thioesterase 8; Ubiquitin thiolesterase 8; Ubiquitin-specific-processing protease 8; UBP8_HUMAN; UBPY; USP8; hUBPy; ubiquitin isopeptidase Y; ubiquitin thioesterase 8; deubiquitinating enzyme 8; ubiquitin thiolesterase 8; ubiquitin-specific-processing protease 8
Gene ID : 9101
mRNA Refseq : NM_001128610
Protein Refseq : NP_001122082
MIM : 603158
UniProt ID : P40818
Chromosome Location : 15q21.1
Pathway : Endocytosis, organism-specific biosystem; Endocytosis, conserved biosystem; ErbB2/ErbB3 signaling events, organism-specific biosystem; Internalization of ErbB1, organism-specific biosystem
Function : SH3 domain binding; cysteine-type endopeptidase activity; peptidase activity; protein binding; ubiquitin thiolesterase activity

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