Active Recombinant Rat Csf2 protein(Ala18-Lys144), hFc-tagged

• Cat.No.: Csf2-553R
• Product Overview: Recombinant Rat CSF2 (P48750) (Ala18-Lys144) was expressed in HEK293 with Fc region of Human IgG1 at the N-terminus.

Specification

• Source: HEK293
• Species: Rat
• Tag: N-hFc
• Protein length: Ala18-Lys144
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured in a cell proliferation assay using FDC-P1 cells. The ED50 for this effect is typically 1-5 ng/mL.
• Molecular Mass: The recombinant rat CSF2 comprises 387 amino acids and predicts a molecular mass of 43 kDa. The apparent molecular mass of the recombinant protein is approximately 43-47 and 36 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Csf2 colony stimulating factor 2 (granulocyte-macrophage) [ Rattus norvegicus ]
• Official Symbol: Csf2
• Synonyms: CSF2; colony stimulating factor 2 (granulocyte-macrophage); granulocyte-macrophage colony-stimulating factor; CSF; colony-stimulating factor; Gmcsf; Gm-csf
• Gene ID: 116630