Recombinant Human NPM1, His-tagged

• Cat.No.: NPM1-54H
• Product Overview: Full-length recombinant human NPM1 protein was expressed in E.coli cells using an N-terminal His tag.

Specification

• Description: NPM1 or nucleophosmin (nucleolar phosphoprotein B23, numatrin) is a ubiquitously expressed nucleolar protein that shuttles between the nucleus and cytoplasm which is implicated in multiple functions, including ribosomal protein assembly and transport, control of centrosome duplication, and regulation of the tumor suppressor ARF NPM1 mutations that relocalize NPM1 from the nucleus into the cytoplasm are associated with development of acute myeloid leukemia (AML). NPM1 is a target of CDK2/cyclin E in the initiation of centrosome duplication. NPM1 is involved in several processes including regulation of the ARF/p53 pathway. NPM1-ALK acts as a tumor suppressor by reciprocally inhibiting NPM1-ALK expression.
• Source: E.coli
• Species: Human
• Tag: His
• Form: 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.
• Molecular Mass: ~40 kDa
• Applications: Western Blot
• Storage: Store product at –70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Gene Information

• Gene Name: NPM1 nucleophosmin (nucleolar phosphoprotein B23, numatrin) [ Homo sapiens ]
• Official Symbol: NPM1
• Synonyms: NPM1; nucleophosmin (nucleolar phosphoprotein B23, numatrin); nucleophosmin; B23; NPM; nucleolar phosphoprotein B23; nucleophosmin/nucleoplasmin family; member 1; numatrin; nucleolar protein NO38; nucleophosmin/nucleoplasmin family, member 1; MGC104254
• Gene ID: 4869
• mRNA Refseq: NM_001037738
• Protein Refseq: NP_001032827
• MIM: 164040
• UniProt ID: P06748
• Chromosome Location: 5q35.1
• Pathway: Aurora B signaling, organism-specific biosystem; BARD1 signaling events, organism-specific biosystem; Cell Cycle, organism-specific biosystem; Chromosome Maintenance, organism-specific biosystem; Deposition of New CENPA-containing Nucleosomes at the Centromere, organism-specific biosystem; Disease, organism-specific biosystem; HIF-1-alpha transcription factor network, organism-specific biosystem
• Function: NF-kappaB binding; NF-kappaB binding; RNA binding; Tat protein binding; histone binding; nucleic acid binding; protein binding; protein heterodimerization activity; protein homodimerization activity; protein kinase binding; ribosomal large subunit binding; ribosomal small subunit binding; transcription coactivator activity; unfolded protein binding; unfolded protein binding