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Recombinant Mouse Interleukin 7

Cat. No.: Il7-77M
Product Overview: Recombinant Mouse Interleukin-7 produced inE.Coliis a single, non-glycosylated polypeptide chain containing 130 amino acids and having a molecular mass of 14897 Dalton. The IL-7 is purified by proprietary chromatographic techniques.
Description: Interleukin-7 is a potent lymphoid cell growth factor produced primarily by stromal cells. IL-7 has been shown to support the proliferation and differentiation of pre B- and early T cells as well as displaying a biological effect on cells of NK and myeloid lineages.
Source: Escherichia Coli.
Amino Acid Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Glu-Cys-His-Ile.
Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Purity: Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Formulation: Lyophilized from a concentrated (1 mg/ml) solution in water containing no additives.
Solubility: It is recommended to reconstitute the lyophilized Interleukin 7 in sterile 18 MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Specific Activity: The ED50as determined by the dose-dependant stimulation of murine 2E8 cells is < 0.2 ng/ml, corresponding to a Specific Activity of 2 x 106 IU/mg.
Protein Content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.48 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of IL-7 as a Reference Standard.
Stability: Lyophilized Interleukin-7 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL7 should be stored at 4°Cbetween 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Gene Name: Il7 interleukin 7 [ Mus musculus ]
Synonyms: Il7; interleukin 7; Il-7; hlb368; MGC129342; A630026I06Rik
Gene ID: 16196
mRNA Refseq: NM_008371
Protein Refseq: NP_032397
UniProt ID: P10168
Chromosome Location: 3 A1; 3 6.6 cM
Pathway: Cytokine-cytokine receptor interaction; Hematopoietic cell lineage; Jak-STAT signaling pathway
Function: cytokine activity; cytokine receptor binding; growth factor activity; interleukin-7 receptor binding

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