Active Recombinant Human IFNG, His-tagged

• Cat.No.: IFNG-24H
• Product Overview: Recombinant Human IFNG was produced in the endosperm tissue of barley grain (Hordeum vulgare).

Specification

• Description: IFN-gamma, a type II interferon and an acid-labile interferon, is mainly produced by CD4 and CD8 T-lymphocytes and natural killer cells activated by antigens, mitogens or alloantigens. IFN-gamma shows no significant homology with IFN-beta or IFN-alpha. IFN-gamma has antiviral and antiparasitic activities and exhibits also immunoregulatory and proinflammatory activities. IFN-gamma induces cytokine production and promotes antigen presentation by up regulating the expression of MHC proteins as well as other antigen proteins. In contrast to other interferons, which are mostly antiviral, the main activity of IFN-gamma seems to be immunomodulatory. Human IFN-gamma is species-specific and is only biologically active in human and primate cells.
• Source: Barley grain (Hordeum vulgare)
• Species: Human
• Tag: His
• Form: Sterile filtered through a 0.2 μm filter. Lyophilized from PBS pH 7.2
• Bio-activity: Recombinant human IFN-gamma was determined by its dose dependent cytostatic effect, in the presence of TNF-alpha, on HT-29 (human carcinoma) cells. The ED50 for this effect is typically below 0.2 ng/ml corresponding to specific activity of >5 x 10e6 U/mg. Optimal concentration should be determined for specific applications and cell lines.
• Molecular Mass: Recombinant human IFN gamma contains 143 amino acids and a 16 a.a. Histidine-based tag for a total length of 159 a.a. and has a predicted molecular mass of 18.8 kDa. As of glycosilation, the recombinant protein migrates with an apparent molecular mass of 20-25 kDa in SDS-PAGE.
• Endotoxin: Endotoxin level is less than 0.005ng per μg of product (0.05EU/μg).
• Purity: Greater than 95% by SDS-PAGE gel analysis.
• Storage: The lyophilized protein, though stable at room temperature for few weeks, is best stored at -20°C. Reconstituted protein should be used immediately or stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.
• Reconstitution: Always centrifuge the vial before opening. It is recommended to reconstitute the lyophilized protein in sterile water to a concentration of no less than 100 μg/ml. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

Gene Information

• Gene Name: IFNG interferon, gamma [ Homo sapiens ]
• Official Symbol: IFNG
• Synonyms: IFNG; interferon, gamma; interferon gamma; IFN-gamma; immune interferon; IFG; IFI
• Gene ID: 3458
• mRNA Refseq: NM_000619
• Protein Refseq: NP_000610
• MIM: 147570
• UniProt ID: P01579
• Chromosome Location: 12q14
• Pathway: ATF-2 transcription factor network, organism-specific biosystem; African trypanosomiasis, organism-specific biosystem; African trypanosomiasis, conserved biosystem; Allograft rejection, organism-specific biosystem; Allograft rejection, conserved biosystem; Amoebiasis, organism-specific biosystem; Amoebiasis, conserved biosystem
• Function: cytokine activity; interferon-gamma receptor binding

Reviews

12/17/2022

It is analyzed by X-ray crystallography or other crystallization techniques and has good crystallization properties.

06/10/2022

Easy to attach to the surface of cells or materials.

04/02/2022

IFNG products have a complete range of specifications for experimental designs of different sizes and needs.

Q&As

What is the role of IFNG in tumor immunotherapy?

IFNG plays an important role in tumor immunotherapy, which can activate the immune response of tumor cells, promote apoptosis of tumor cells and inhibit the proliferation of tumor cells.

What is the current progress in drug development for IFNG?

The development of drugs for IFNG is currently in clinical trials, including monoclonal antibodies against IFNG receptors and inhibitors against IFNG signaling pathways.

How is IFNG related to muscle wasting?

Studies have shown that IFNG can inhibit the occurrence of muscle atrophy, but the specific mechanism is not clear.

How can IFNG be used as a biomarker in clinical practice?

In clinical practice, the expression level of IFNG can be measured to predict the progression and prognosis of the disease, and to guide the choice of treatment regimen.

Are there other coding variants in the IFNG gene?

Yes, there are other coding variants in the IFNG gene that may have different functions and expression patterns.

Is the expression of IFNG affected by environmental factors?

Such as nutritional status, inflammation levels, etc. Yes, the expression of IFNG is affected by environmental factors, such as nutritional status, inflammation level, etc.